Mitochondrial trifunctional protein (TFP) subunit alpha (HADHA)
monoclonal antibody
|
Catalog No. MS702
|
|
|
$325.00 - 100 µg
|
|
|
UniProt Number:
|
P40939
|
|
Alternate Names:
|
Trifunctional enzyme subunit alpha, TP-alpha, 78 kDa gastrin-binding protein, HADH
|
|
Structure and Function:
|
The Mitochondrial trifunctional protein (TFP) alpha subunit (HADHA) is a 79 kDa enzyme which has two enzymatic functions, the hydration of enoyl-CoA and the dehydrogenation of 3-hydroxyacyl CoA.
|
|
Disease Associations:
|
Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) [MIM:609015], long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) [MIM:609016], and maternal acute fatty liver of pregnancy (AFLP) [MIM:609016].
|
|
|
|
Product Specifications
|
|
|
Applications:
|
Western blotting, Immunocytochemistry, Immunoprecipitation, In-Cell ELISA, Flow Cytometry
|
|
Species Reactivity:
|
human
|
|
Host Species:
|
mouse
|
|
Isotype:
|
IgG2b, κ
|
|
Clone ID:
|
2C2AA5
|
|
Concentration:
|
1.0 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
|
|
Suggested Working Concentration:
|
0.5 µg/mL for Western blotting
4 µg/mL for Immunocytochemistry
1 µg/mL for In-Cell ELISA (0.1 µg/well)
1 µg/mL for Flow Cytometry
|
|
Storage Conditions:
|
Store at 4°C. Do not freeze.
|
|
Country of Origin:
|
USA
|
Top
|
|
|
|
|
ICC Images
(click to enlarge)
Figure 1. Immunocytochemistry image of MS702 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (2C2AA5, 4 µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (green) AlexaFluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
|
WB Images
(click to enlarge)
Figure 2. A Western blot image of MS702 with human heart mitochondria (HHM), and human liver mitochondria (HLM). An anti-Complex II 30kDa subunit antibody has been used as a control. MS702 also immunocaptures both the alpha and beta subunits of TFP.
|
Flow Images
(click to enlarge)
Figure 3. HeLa cells were stained with 1 µg/mL TFP (HADHA) antibody (MS702) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
|
Top
|
|
|
|
|
Have you published studies using this product?
Let us know by filling out this form and receive a $100 discount on your next order.
Top
|
|
|
|
CART
