Mitochondrial trifunctional protein (TFP) subunit alpha (HADHA)

monoclonal antibody

Catalog No. MS702

$325.00 - 100 µg

UniProt Number: P40939
Alternate Names: Trifunctional enzyme subunit alpha, TP-alpha, 78 kDa gastrin-binding protein, HADH
Structure and Function: The Mitochondrial trifunctional protein (TFP) alpha subunit (HADHA) is a 79 kDa enzyme which has two enzymatic functions, the hydration of enoyl-CoA and the dehydrogenation of 3-hydroxyacyl CoA.
Disease Associations: Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) [MIM:609015], long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) [MIM:609016], and maternal acute fatty liver of pregnancy (AFLP) [MIM:609016].


Product Specifications
Applications: Western blotting, Immunocytochemistry, Immunoprecipitation, In-Cell ELISA, Flow Cytometry
Species Reactivity: human
Host Species: mouse
Isotype: IgG2b, κ
Clone ID: 2C2AA5
Concentration: 1.0 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
Suggested Working Concentration: 0.5 µg/mL for Western blotting
4 µg/mL for Immunocytochemistry
1 µg/mL for In-Cell ELISA (0.1 µg/well)
1 µg/mL for Flow Cytometry
Storage Conditions: Store at 4°C. Do not freeze.
Country of Origin: USA



ICC Images

(click to enlarge)

Figure 1. Immunocytochemistry image of MS702 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (2C2AA5, 4 µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (green) AlexaFluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.

WB Images

(click to enlarge)

Figure 2. A Western blot image of MS702 with human heart mitochondria (HHM), and human liver mitochondria (HLM). An anti-Complex II 30kDa subunit antibody has been used as a control. MS702 also immunocaptures both the alpha and beta subunits of TFP.

Flow Images

(click to enlarge)

Figure 3. HeLa cells were stained with 1 µg/mL TFP (HADHA) antibody (MS702) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.



Downloadable Documents

   Technical Data Sheet

   Western blotting Protocol

   Immunocytochemistry Protocol

   Immunocapture Protocol

   In-Cell ELISA Protocol

   Flow Cytometry Protocol

   MSDS Sodium Azide



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