Mitochondrial trifunctional protein (TFP) subunit beta (HADHB)
monoclonal antibody
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Catalog No. MS733
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$325.00 - 100 µg
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This antibody can also be purchased as part of a Sample Pack.
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UniProt Number:
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P55084
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Alternate Names:
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TP-beta, Trifunctional enzyme subunit beta,
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Structure and Function:
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The Mitochondrial trifunctional protein (TFP) beta subunit (HADHB), a 47 kDa enzyme, performs thiolytic cleavage of 3-ketoacyl-CoA substrates.
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Disease Associations:
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Defects in HADHB are a cause of trifunctional protein deficiency (TFP deficiency) [MIM:609015].
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Product Specifications
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Applications:
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Immunoprecipitation, Immunocytochemistry, Western blotting, In-Cell ELISA
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Species Reactivity:
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human
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Host Species:
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mouse
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Isotype:
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IgG1, κ
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Clone ID:
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5C3AB7
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Concentration:
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1 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
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Suggested Working Concentration:
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1 µg/mL for Immunocytochemistry
0.5 µg/mL for Western blotting
1 µg/mL for In-Cell ELISA (0.1 µg/well)
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Storage Conditions:
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Store at 4°C. Do not freeze.
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Country of Origin:
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USA
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IP Images
(click to enlarge)
Figure 1. As an IP antibody, MS733 works with human samples.
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ICC Images
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Figure 2. Immunocytochemistry image of MS733 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (5C3AB7, 1 µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria. Suggested working concentration is 1 ug/ml.
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WB Images
(click to enlarge)
Figure 3. As a Western blot antibody, MS733 works with HepG2 (human) cells.
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