MetaPath™ MitoDisease 4-Plex Dipstick Array

Catalog No. MSX31

$545.00 - 30 tests
$995.00 - 90 tests


Product Description

This novel array allows for the simultaneous quantification of 4 key enzymes whose expression is down-regulated in different mitochondrial disease patients: Complex I, Complex IV, PDH and Frataxin. The array is comprised of 4 monoclonal capture antibodies striped onto a dipstick, and the immunocaptured sample is detected using 4 monoclonal detector antibodies that recognize different epitopes on the target enzymes. This array is very rapid (total assay time including sample prep is less than 1 hour) and very sensitive, and as with all MitoSciences' assays, isolation of mitochondria is not required.

This array is suitable for testing a variety of sample types, including cultured cells and tissue from cheek swabs. Not only is it useful for measuring samples that are deficient in the 4 enzymes due to genetic mutations, it is also useful for measuring changes in the expression of the enzymes due to drug treatments or other manipulations.

Learn more about Dipstick Protein Quantity Assays >



 
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Product Specifications
 
Species Reactivity: human
Storage Conditions: Store dipsticks and gold-conjugated secondary antibody at room temperature out of direct sunlight in their provided containers.

Store buffers at 4°C, or at -20°C for long term storage.
Country of Origin: USA


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Figure 1


(click to enlarge)

Figure 1. A typical standard curve for MSX31 using HepG2 cell extract. We suggest using 7 to 8 dipsticks for covering the working range. In this example, the maximum sample load should be 5 µg linear regression above which non-linear regression should be used.

Figure 2


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Figure 2. Examples of dipsticks from (a) normal fibroblast cells (b) rho0 fibroblast cells which lack mtDNA and hence complexes I and IV only (c) a fibroblast sample from a patient exhibiting a specific deficiency in complex I (d) pure frataxin and (e) buffer control with containing no sample. Data from these sticks was collected using a simple flatbed scanner and ImageJ software.


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Downloadable Documents


   Technical Data Sheet

   Protocol

   Sample Preparation Guide


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Companion Products:
• MetaPath™ Fatty Acid Oxidation
  4-Plex Dipstick Array
(cat.#MSX32)

• Dipstick Reader (cat.#MS1000)




Click here for a video demonstration of a dipstick assay.



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