Products for Immunocytochemistry
- Research Kits
- Individual Antibodies
Overview
MitoSciences provides antibodies for the detection of mitochondrial proteins by immunocytochemistry. Antibodies are available against individual subunits of OXPHOS complexes in addition to other proteins of the inner mitochondrial membrane. A series of antibodies is available against subunits of the pyruvate dehydrogenase complex (PDH). Also available is a kit (MS602) containing antibodies which have different isotypes thereby allowing simultaneous immunocytochemical analaysis of multiple OXPHOS proteins.
Research Kits
MS602 MitoProfile® OXPHOS/PDH Immunocytochemistry Kit
A cocktail of 220 µg antibodies comprised of six mAbs packaged individually. Each antibody is against a labile subunit of each of the 5 OXPHOS complexes and PDH. Each mAb is provided in a 50 µl aliquot at a 200x concentration, to allow reconstitution of each mAb in 10 ml working volume.
MS602a MitoProfile® OXPHOS/PDH Immunocytochemistry Kit
Includes the same components as MS602 (above), but additionally contains 60 µg mAb to porin, and 120 µg to Complex V α subunit, and the required secondary antibodies for simultaneous 2 dye staining of samples.
MSA07 MitoProfile® Apoptosis Detection Kit for Immunocytochemistry
MSA07 detects apoptotic cells with high specificity. It contains an anti-cytochrome c monoclonal antibody (50 µg) and the appropriate isotype-specific secondary antibody (50 µg) which has a fluorescein fluorophore conjugated to it. The kit also contains an anti-Complex Vα monoclonal antibody (50 µg) and the appropriate Texas Red fluorophore-conjugated isotype-specific secondary antibody (50 µg). Complex V serves as a mitochondrial marker in healthy and apoptotic cells.
Individual Antibodies
MS103 Complex I subunit GRIM-19 monoclonal antibody
100 µg of monoclonal antibody reactive against the 19 kDa Complex I (NADH dehydrogenase) subunit GRIM-19 for immunocytochemistry. Interestingly GRIM-19 is also present in the nucleus and cytoplasm. Its expression is necessary for interferonβ/retinoic acid induced tumor mortality.
MS107 Complex I subunit NDUFB4 monoclonal antibody
100 µg of monoclonal antibody reactive against the 15 kDa Complex I (NADH dehydrogenase) subunit NDUFB4. MS107 also labels in immunocytochemical applications. Formerly listed as likely subunit NDUFA6.
MS110 Complex I subunit NDUFS3 monoclonal antibody
100 µg of monoclonal antibody reactive against the 30 kDa Complex I (NADH dehydrogenase) NDUFS3 subunit.
MS203 Complex II subunit 30 kDa Ip monoclonal antibody
100 µg of monoclonal antibody reactive against the Complex II 30 kDa Ip subunit in immunocytochemical applications.
MS204 Complex II subunit 70 kDa Fp monoclonal antibody
100 µg of monoclonal antibody reactive against the Complex II 70 kDa Fp subunit in immunocytochemical applications.
MS304 Complex III subunit Core 2 monoclonal antibody
100 µg of monoclonal antibody reactive against the 47 kDa complex III Core 2 protein for immunocytochemistry.
MS404 Complex IV subunit I monoclonal antibody
100 µg of monoclonal antibody reactive against the 57 kDa COX (Complex IV) subunit I for immunocytochemistry.
MS405 Complex IV subunit II monoclonal antibody
100 µg of monoclonal antibody reactive against the 26 kDa COX (Complex IV) subunit II for immunocytochemistry.
MS408 (alternate to 407) Complex IV subunit IV monoclonal antibody
100 µg of monoclonal antibody reactive against the 18 kDa COX (Complex IV) subunit IV. MS408 also labels COX IV in immunocytochemical applications. MS407 also binds COX IV. Reacts only with human and bovine, but better than MS407 for immunocytochemistry in these species.
MS409 Complex IV subunit Va monoclonal antibody
100 µg of monoclonal antibody reactive against the 12 kDa COX (Complex IV) subunit Va for immunocytochemistry.
MS502 ATP synthase (Complex V) subunit α monoclonal antibody
100 µg of monoclonal antibody reactive against the 55 kDa F1F0 ATP synthase (Complex V) subunit α for immunocytochemistry.
MS503 ATP synthase (Complex V) subunit β monoclonal antibody
100 µg of monoclonal antibody reactive against the 52 kDa F1F0 ATP synthase (Complex V) subunit β for immunocytochemistry.
MS504 ATP synthase (Complex V) subunit d monoclonal antibody
100 µg of monoclonal antibody reactive against the 19 kDa F1F0 ATP synthase (Complex V) subunit d for immunocytochemistry.
MS505 ATP synthase (Complex V) subunit OSCP monoclonal antibody
100 µg of monoclonal antibody reactive against the 21 kDa F1F0 ATP synthase (Complex V) OSCP subunit for immunocytochemistry.
MS507 ATP synthase (Complex V) subunit α monoclonal antibody; with broad cross-reactivity
100 µg of monoclonal antibody reactive against the 55 kDa F1F0 ATP synthase (Complex V) α subunit. Reacts with animal and plant subunits.
MS508 ATP synthase (Complex V) subunit F6 monoclonal antibody
100 µg of monoclonal antibody reactive against the 9 kDa F1F0 ATP synthase (Complex V) F6 subunit.
MS701 NNT Monoclonal Antibody
Nicotinamide nucleotide transhydrogenase (NNT) is a mitochondrial inner membrane protein catalyzing the interconversion of NADH and NADPH within the matrix space of the organelle. It couples this reaction to proton translocation across the inner membrane. In the presence of an electrochemical proton gradient the production of NADPH is strongly favored. NADPH is required in the mitochondrial matrix to keep the ratio GSH:GSSG high via the NADPH dependent glutathione reductase. Thus NNT has an important role to play in defences against oxidative stress produced by the organelle. A novel, unexpected role of NNT is in insulin secretion. The absence of NNT prevents glucose from closing the K+ATP channel and from opening the Ca++ channel in pancreatic beta cells. These defective beta cells also show a profound increase inROS.
MSA02 ANT monoclonal antibody
100 µg of monoclonal antibody reactive against the 32 kDa adenine nucleotide translocase (ANT) for immunocytochemistry.
MSA04 Cyclophilin D monoclonal antibody
100 µg of monoclonal antibody reactive against the 21 kDa human cyclophilin'D' subunit (P30405) for immunocytochemistry.
MSA05 Porin monoclonal antibody
100 µg of monoclonal antibody reactive against the 39 kDa human porin (VDAC1) for immunocytochemistry.
MSA06 Cytochrome c monoclonal antibody
100 µg of monoclonal antibody reactive against the 12 kDa cytochrome c for immunocytochemistry.
MSA09 AIF (Apoptosis-Inducing Factor) monoclonal antibody
100 µg of monoclonal antibody reactive against the 67 kDa AIF, suitable for use in for Western blotting and immunocytochemistry applications. Human heart mitochondria are included as a Western blotting control.
MSM02 Mitofilin monoclonal antibody
100 µg of monoclonal antibody against mitofilin, suitable for use in Western Blotting and Immunocytochemistry. Human heart mitochondria are also included as a Western Blotting control. The mitofilin monoclonal antibody reacts against 2 isoforms (about 90 and 91kDa) in Western blots with human heart mitochondria. A single form of mitofilin is detected in Western blots with human liver mitochondria.
MSP03 PDH subunit α monoclonal antibody
100 µg of monoclonal antibody reactive against the 44 kDa PDH subunit E1α for immunocytochemistry.
MSP05 PDH subunit E2 monoclonal antibody
100 µg of monoclonal antibody reactive against the 66 kDa PDH E2 subunit for immunocytochemistry.
MSP06 PDH subunit E2/E3bp monoclonal antibody
100 µg of monoclonal antibody reactive against both the 66 kDa PDH E2 subunit and the 54 kDa E3 binding protein in immunocytochemical studies. Preliminary data suggest that this antibody recognizes the bound lipoic acid on both proteins.
MSP07 PDH subunit α monoclonal antibody
100 µg of monoclonal antibody reactive against the 44 kDa PDH subunit E1α for immunocytochemistry.
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MSA07 Apoptosis Detection Kit
Apoptotic cells which have released mitochondrial cytochrome c into the cytosol can be differentiated from non apoptotic cells which still retain cytochrome c in their mitochondria by fluorescence microscopy. The kit also contains an anti-Complex V α monoclonal antibody (50 µg) and the appropriate isotype-specific secondary antibody (50 µg) which has a Texas Red fluorophore conjugated to it.
Complex V is an inner mitochondrial membrane protein and serves as a mitochondrial marker since it is not released into the cytoplasm during apoptosis, unlike cytochrome c. Thus one is able to observe cytochrome c and a mitochondrial marker (Complex Vα) simultaneously by fluorescence microscopy using MSA07. Buffers and blocking solution for immunocytochemical analysis are also included in the kit.
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