Products for Immunocapture


- Research Kits
- Individual Antibodies

Overview

MitoSciences provides antibodies for the purification of mitochondrial proteins by immunoprecipitation. These antibodies are available free in solution or preconjugated to agarose beads.

Specifically antibodies are available for the isolation of each of the OXPHOS complexes I, II, III, IV and V in addition to other proteins of the inner mitochondrial membrane. An antibody is also available for the isolation of the pyruvate dehydrogenase complex (PDH).

 

Research Kits


MS101 MitoProfile® Complex I Immunocapture Kit
250 µg, 500 µg or 750 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~60 µg, ~120 µg or ~180 µg respectively of Complex I (NADH dehydrogenase) from heart mitochondria.

MS201 MitoProfile® Complex II Immunocapture Kit
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 g, ~26 g or ~39 g respectively of Complex II from heart mitochondria.

MS301 MitoProfile® Complex III Immunocapture Kit
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex III from heart mitochondria.

MS401 MitoProfile® Complex IV Immunocapture Kit
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 g, ~50 g or ~75 g respectively of Complex IV from heart mitochondria.

MS402 MitoProfile® Complex IV Immunocapture Kit for Rodent
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 g, ~50 g or ~75 g respectively of Complex IV from heart mitochondria. The Complex IV immunocapture kit allows isolation of the cytochrome c oxidase complex (E.C. 1.9.3.1) from small amounts of rat or mouse tissue.

MS501 MitoProfile® ATP synthase (Complex V) Immunocapture Kit
250 µg, 500 µg or 750 µg of monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 g, ~50 g or ~75 g respectively of Complex V from heart mitochondria.

MSA01 MitoProfile® ANT Immunocapture Kit
250 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ANT from heart mitochondria.

MSP01 MitoProfile® PDH Immunocapture Kit
250 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 µg of PDH complex from heart mitochondria.

 

Individual Antibodies


MS101c Complex I Immunocapture monoclonal antibody
100 µg monoclonal antibody which can immunocapture ~25 µg of Complex I (NADH dehydrogenase) from heart mitochondria.

MS201c Complex II Immunocapture monoclonal antibody
100 µg monoclonal antibody which can immunocapture ~5 µg of Complex II from heart mitochondria.

MS301c Complex III Immunocapture monoclonal antibody
100 µg monoclonal antibody which can immunocapture ~10 µg of Complex III from heart mitochondria.

MS401c Complex IV Immunocapture monoclonal antibody
100 µg monoclonal antibody which can immunocapture ~10 µg of Complex IV from heart mitochondria.

MS501c ATP synthase (Complex V) Immunocapture monoclonal antibody
100 µg monoclonal antibody which can immunocapture up to 25 µg of Complex V from heart mitochondria.

MSA01c ANT immunocapture monoclonal antibody
100 µg monoclonal antibody which can immunocapture ANT from heart mitochondria.

MSP01c PDH Immunocapture monoclonal antibody
100 µg monoclonal antibody which can immunocapture PDH from heart mitochondria.



Featured           
Product
MS101 Complex I Immunocapture Kit

This kit allows for isolation of Complex I from small amounts of tissue. This facilitates subsequent analysis of post-translational modifications (including oxidative damage) by proteomic approaches or antibody detection of these modifications.

Uses for this kit include but are not limited to examining alterations of Complex I subunits in inherited mitochondrial diseases, Parkinson's disease, Alzheimer's disease, ALS, schizophrenia, and aging.
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