Frataxin Protein Quantity Microplate Assay Kit

Catalog No. MSF41

$425.00 - 96 tests


Product Description

A 96-well plate assay for rapidly determining the amount of frataxin in a human sample. Frataxin is immunocaptured within the wells. The quantity of protein is measured by adding a second frataxin specific antibody that is labeled with horseradish peroxidase. This peroxidase changes the substrate from colorless to blue. The rate of color development is proportional to the amount of protein captured in the well and can be monitored at 600 nm. Alternatively the assay can be terminated, at a user defined time, by the addition of 1N HCl (not supplied) and the assay performed as an end point measurement at 450 nm.



 
Data
 
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Product Specifications
 
Species Reactivity: human
Storage Conditions: 4° C.
Country of Origin: USA


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Figure 1


(click to enlarge)

Figure 1. The quantity of frataxin captured in each well is proportional to the amount of horseradish peroxidase activity within each well. The quantity is the change in absorbance at 600 nm/minute/amount of sample loaded into the well. Examine the linear rate of increase in absorbance at 600 nm with time. As shown, the rate/slope is calculated between these time points.

For the control or normal sample, the rate versus amount loaded is plotted as a straight line in the linear region of the assay as shown. Compare the rates of the control (normal) sample and with the rate of the null (background) and with your unknowns, experimental or treated samples to get the relative amount of frataxin.


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Specifications
 
Data
 
 
Citations

Downloadable Documents


   Technical Data Sheet

   Protocol

   Sample Preparation Guide

   Frataxin Brochure

   MSDS Sodium Azide


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Specifications
 
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