Frataxin monoclonal antibody
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Catalog No. MSF01
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$325.00 - 100 µg
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Product Specifications
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Applications:
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Western blotting, Immunocytochemistry, Flow Cytometry
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Species Reactivity:
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human
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Host Species:
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mouse
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Isotype:
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IgG1, κ
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Clone ID:
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18A5DB1
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Concentration:
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1.0 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
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Suggested Working Concentration:
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3-5 µg/mL for Western blotting (a chemiluminescent detection system is highly recommended)
0.5 µg/mL for Immunocytochemistry
1 µg/mL for Flow Cytometry
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Storage Conditions:
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Store at 4°C. Do not freeze.
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Country of Origin:
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USA
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WB Images
(click to enlarge)
Figure 1. Recombinant Human Frataxin (lane 1) detected with (MSF01) anti-Frataxin antibody.
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ICC Images
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Figure 2. Mitochondrial localization of Frataxin in normal human fibroblasts visualized by immunocytochemistry using anti-frataxin mAb 18A5DB1 (MSF01). Cultured human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with MSF01 (0.5 µg/ml) followed by an AlexaFluor® 594-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).
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Flow Images
(click to enlarge)
Figure 3. HL-60 cells were stained with 1 µg/mL Frataxin antibody (MSF01) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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Published Studies Using This Product:
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Selak et al., 2010. Blood cells from Friedreich ataxia patients harbor frataxin deficiency without a loss of mitochondrial function.
Koeppen et al., 2010. The Neuropathology of Late-Onset Friedreich's Ataxia.
Gakh et al., 2010. Normal and Friedreich ataxia cells express different isoforms of frataxin with complementary roles in iron-sulfur cluster assembly.
Li et al., 2010. Expression of Human Frataxin Is Regulated by Transcription Factors SRF and TFAP2.
Rai et al., 2010. Two new pimelic diphenylamide HDAC inhibitors induce sustained frataxin upregulation in cells from Friedreich's ataxia patients and in a mouse model.
Nadanaciva et al., 2009. Lateral-flow immunoassay for detecting drug-induced inhibition of mitochondrial DNA replication and mtDNA-encoded protein synthesis.
Chou et al., 2008. Pimelic diphenylamide 106 is a slow, tight-binding inhibitor of class I histone deacetylases.
Schmucker et al., 2008. The in vivo mitochondrial two-step maturation of human frataxin.
Long et al., 2008. Mitochondrial localization of human frataxin is necessary but processing is not for rescuing frataxin deficiency in Trypanosoma brucei.
Willis et al., 2008. Lateral-flow immunoassay for the frataxin protein in Friedreich's ataxia patients and carriers.
Li et al., 2008. Iron-dependent regulation of frataxin expression: implications for treatment of Friedreich ataxia.
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