Enzyme Activity ELISA Kits

Our enzyme activity assays involve the immunocapture of the protein of interest first followed by measurement of substrate or product turnover. These employ only monoclonal antibodies that have been screened to isolate the enzyme in active form and in complexes that likely represent their associations in situ. The advantages are many. The enzyme is separated from other proteins that use the or produce the same substrates or products and this allows for simple assays such as utilization of ATP, NADH, pyruvate etc. which are subject to multiple reactions in vivo. For most of the enzymes for which we have activity assay kits, we also provide Sandwich ELISA assays of quantity. By combining the two, it is possible to generate specific activity data. Furthermore, because the enzyme is separated from other cellular components, the post-translational modifications (PTMs) it contains at the time of assay can be measured e.g. by subsequent mass spectrometry or reaction to PTM specific antibodies.

Enzyme Activity ELISA Kits

Microplate Kits
Ideal for high-throughput analyses of tissue and cultured cell samples from a variety of species.
Dipstick Kits
Ideal for situations where a rapid, simple, and sensitive assay solution is desired for quantitative analyses.

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