ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail

Catalog No. MSA12

$425.00 - 180 µg

Product Description

The permeabilization of mitochondrial outer membrane and the subsequent release of cytochrome c and other apoptogenic proteins from mitochondrial intermembrane space into the cytoplasm is considered a hallmark of many apoptotic pathways. Therefore assaying these proteins in mitochondrial and cytoplasmic fractions is of prime interest for many researchers.

MSA12 is a Western blot antibody cocktail that allows for the detection of cytochrome c in cytoplasmic and mitochondria-containing fractions for determining the proportion of released cytochrome c from mitochondria to the cytoplasm from apoptosis. The kit also includes antibodies against a cytoplasmic protein marker, glyceraldehyde-3-phosphodehydrogenase (GAPDH), and 2 mitochondrial markers, pyruvate dehydrogenase subunit E1-alpha (a matrix marker), and ATP synthase subunit alpha (an inner membrane marker). This set of control markers allows for the monitoring and/or optimization of the permeabilization conditions.


Product Specifications
Targets: • Cytochrome c
• Glyceraldehyde-3-phosphodehydrogenase (GAPDH)
• Pyruvate dehydrogenase subunit E1-alpha
• ATP synthase subunit alpha
Species Reactivity: human
Storage Conditions: 4° C. Do not freeze.
Country of Origin: USA


Figure 1

(click to enlarge)

Figure 1. In this experiment, apoptosis was induced in Jurkat and 143B osteosarcoma cells by FAS and also by treatment with staurosporine (HeLa cells were also treated, but only with STS). Mitochondrial and cytoplasmic fractions were isolated (using kit Cell Fractionation Kit MS861) and probed using MSA12.

As is clear from the gels, cytochrome c has translocated partially in FAS-induced cells and STS-treated osteosarcoma cells, and almost completely in STS-treated Jurkat and HeLa cells. The three control targets allow for verification of the "cleanness" of the cell fractionation.



Downloadable Documents

   Technical Data Sheet


   MSDS Sodium Azide



Published Studies Using This Product: Plant et al., 2009. Absence of caspase-3 protects against denervation-induced skeletal muscle atrophy.

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