Cytochrome c Protein Quantity Microplate Assay Kit

Catalog No. MSA41

$425.00 - 96 tests


Product Description

A 96-well microplate assay that is used to determine the amount of cytochrome c in a human, mouse, rat or bovine sample. Cytochrome c is immunocaptured within the wells and the amount is determined by adding a cytochrome c specific antibody conjugated with horseradish peroxidase. This peroxidase changes the substrate from colorless to blue at 600 nm. This reaction takes place in a time dependent manner proportional to the amount of protein captured in the wells. The rate of development of blue color can be followed at 600 nm or the reaction can be stopped at a user defined time by the addition of 100 Ál of 1.5N HCl to each well (not supplied) and read at 450 nm. Included for the performance of this quantity assay are buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.



 
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Product Specifications
 
Species Reactivity: human, mouse, rat, bovine
Storage Conditions: Store detergent at room temperature.

All other tubes and the covered microplate should be stored at 4°C.
Country of Origin: USA


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Figure 1


(click to enlarge)

Figure 1. Relative amounts of cytochrome c in samples derived from the MitoSciences' MSA41 Kit. Results: Cytochrome c is detected in sample [C + 2] - the cytoplasmic (C) fraction only after apoptosis has been initiated (2) and the cell outer membrane has been permeabilized with apoptosis kit detergent (+).


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Specifications
 
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Downloadable Documents


   Technical Data Sheet

   Protocol

   Sample Preparation Guide

   MSDS Sodium Azide


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Specifications
 
Data
 
Documentation
 

Published Studies Using This Product: Eliseev et al., 2009. Cyclophilin D interacts with Bcl2 and exerts an anti-apoptotic effect.


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