ApoTrack™ Cytochrome c Apoptosis ICC Antibody Kit

Catalog No. MSA07

$425.00 - 200 µg

Product Description

Apoptotic cells which have released mitochondrial cytochrome c into the cytosol can be differentiated from non apoptotic cells which still retain cytochrome c in their mitochondria by fluorescence microscopy.

This kit contains a cytochrome c monoclonal antibody (50 µg) and an isotype-specific secondary antibody (50 µg) which has a fluorescein fluorophore conjugated to it. The kit also contains an ATP synthase V subunit alpha monoclonal antibody (50 µg) and the appropriate isotype-specific secondary antibody (50 µg) which has a Texas Red fluorophore conjugated to it.

ATP synthase is an inner mitochondrial membrane protein and serves as a mitochondrial marker since it is not released into the cytoplasm during apoptosis, unlike cytochrome c. Thus one is able to observe cytochrome c and a mitochondrial marker (ATP synthase subunit alpha) simultaneously by fluorescence microscopy using MSA07. Buffers and blocking solution for immunocytochemical analysis are also included in the kit.


Product Specifications
Targets: • Cytochrome c
• ATP synthase subunit alpha
• goat anti-mouse IgG2a-FITC
• goat anti-mouse IgG2b-TXRD
Species Reactivity: human
Storage Conditions: 4° C. Do not freeze.
Country of Origin: USA



Figure 1

Figure 1. Staurosporine-treated HeLa cells analyzed with anti-cytochrome c antibody. The white arrow indicates cytochrome c release from mitochondria.

Figure 2

Figure 2. Staurosporine-treated HeLa cells analyzed with anti-ATP synthase subunit alpha antibody.

Figure 3

Figure 3. Figures 1 & 2 merged.



Downloadable Documents

   Technical Data Sheet


   MSDS Sodium Azide



Published Studies Using This Product: Zhang et al., 2010. The mitochondria pathway of anesthetic isoflurane induced-apoptosis.

Wood-Kaczmar et al., 2008. PINK1 is necessary for long term survival and mitochondrial function in human dopaminergic neurons.

Liekens et al., 2007. The nucleotide analog cidofovir suppresses basic fibroblast growth factor (FGF2) expression and signaling and induces apoptosis in FGF2-overexpressing endothelial cells.

Lagna et al., 2006. BMP-dependent activation of caspase-9 and caspase-8 mediates apoptosis in pulmonary artery smooth muscle cells.


Browse Products By:
Product Search:

or use our     
Product Finder Tool   

Companion Products:
• Cytochrome c monoclonal antibody

• Cytochrome c Apoptosis Cocktail

• Cytochrome c FlexPlex™ Module

• Cytochrome c Protein Quantity
  Microplate Assay Kit

Sales & Customer Support:

[email protected]

© 2004-2011 MitoSciences Inc, an Abcam company. All rights reserved.