MitoSciences: MSFX-16 FlexPlex™ Module: Cytochrome c

Enzymes > Cytochrome c > FlexPlex™ Module: Cytochrome c

FlexPlex™ Module: Cytochrome c

Catalog No. MSFX-16

$125.00 - 1 module (4 strips)

The Cytochrome c FlexPlex™ Module is used to determine the quantity of Cytochrome c in a sample. Cytochrome c is immunocaptured within the wells. The quantity of protein is measured by adding a second Cytochrome c specific antibody that is then labeled with horseradish peroxidase. This peroxidase changes the substrate from colorless to blue. The rate of color development is proportional to the amount of protein captured in the well and can be monitored at 600 nm. Alternatively, the assay can be terminated, at a user defined time, by the addition of 1N HCl (not supplied) and the assay performed as an end point measurement at 450 nm.

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Specifications

Data

Documentation

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Product Specifications
Applications:	analysis of cytochrome c quantity
Species Reactivity:	human, bovine
Range (min. 2x background):	0.6 - 40 µg / well (Example: HepG2)
Av. Intra-assay %CV (n=6):	4.3%
Storage Conditions:	Store at 4°C.
Country of Origin:	USA

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Specifications

Data

Documentation

Citations

Figure 1

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Figure 1. HepG2 titration series. Increasing amounts of HepG2 cells were analyzed using MSFX-16 to demonstrate the potential working range of the assay.

Figure 2

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Figure 2. HL60 titration series. Increasing amounts of HL60 were analyzed using MSFX-16 to demonstrate the potential working range of the assay.

Figure 3

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Figure 3. Human heart tissue titration series. Increasing amounts of human heart tissue homogenate were analyzed using MSFX-16 to demonstrate the potential working range of the assay.

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