MitoSciences: MS432 Complex IV Rodent Enzyme Activity Dipstick Assay Kit

Proteins > Complex IV > Complex IV Rodent Enzyme Activity Dipstick Assay Kit

Complex IV Rodent Enzyme Activity Dipstick Assay Kit

Catalog No. MS432

$325.00 - 30 tests
$595.00 - 90 tests

Product Description

Contains 30 or 90 dipsticks and necessary components to quantify the activity of the cytochrome c oxidase enzyme complex from mouse and rat samples. The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.

Learn more about Dipstick Enzyme Activity Assays >

Specifications

Data

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Product Specifications
Species Reactivity:	mouse, rat
Storage Conditions:	Store dipsticks, gold-conjugated secondary antibody, and Tube 3 at room temperature out of direct sunlight in their provided containers. Store buffers A, B, and C at 4°C. Store Tube 1 and Tube 2 at -20°C.
Country of Origin:	USA

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Specifications

Data

Documentation

Citations

Figure 1

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Figure 1. An example using MS432 to quantify Complex IV enzyme activity in mouse protein extracts

Developed dipsticks from a 1:2 dilution series using a positive control sample and the associated standard curve. Starting material was 100 µg of protein extract.

Figure 2

(click to enlarge)
Figure 2. An example using MS432 to quantify Complex IV enzyme activity in mouse protein extracts

Based on the standard curve, 50 µg of protein extract were loaded onto a dipstick for each sample. The figure shows three developed dipsticks from unknown samples (1-3). The analysis of the signal intensity and interpolation from the standard curve showed that the unknown samples have between 23-87% of normal Complex IV activity levels.

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