MitoSciences: MS444 Complex IV Rodent Enzyme Activity Microplate Assay Kit

Proteins > Complex IV > Complex IV Rodent Enzyme Activity Microplate Assay Kit

Complex IV Rodent Enzyme Activity Microplate Assay Kit

Catalog No. MS444

$425.00 - 96 tests

Product Description

This activity microplate kit is used to determine the activity of cytochrome c oxidase in a mouse sample with speed and simplicity. The COX enzyme is immunocaptured within the wells of the microplate and activity is determined colorimetrically by following the oxidation of reduced cytochrome c by the absorbance change at 550 nm. Included in this kit for performance of the activity assay are buffer, detergent, substrate, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

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Product Specifications
Species Reactivity:	mouse, rat
Storage Conditions:	Buffer, detergent, and microplate should be stored at 4°C, Reagent C (reduced cytochrome c) should be stored at -20°C.
Country of Origin:	USA

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Specifications

Data

Documentation

Citations

Figure 1

(click to enlarge)
Figure 1. To determine the activity in the sample, calculate the slope by using microplate software or by manual calculations using one of the two methods shown. Compare the sample rate with the rate of the control (normal) sample and with the rate of the null (background) to get the relative Complex IV activity. (A)The rate is determined by calculating the gradient of the initial slope over the linear region. (B) The rate is determined by calculating the slope between two points within the linear region.

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