Complex IV Human Specific Activity Microplate Assay Kit

Catalog No. MS443

$495.00 - 96 tests


Product Description

This rapid multiplexing microplate kit is used to determine both the activity and quantity of cytochrome c oxidase in a human sample with greater speed and simplicity. The ratio of the two is a measure of specific activity. The COX enzyme is immunocaptured within the wells of the microplate and the activity is determined by following the oxidation of reduced Cytochrome c colorimetrically by the absorbance change at 550 nm. Subsequently, in these same wells the quantity of the enzyme can then be determined by adding a COX specific detector antibody and the appropriate alkaline phophatase conjugated antibody; this phosphatase changes a substrate from colorless to yellow at 405 nm. This reaction takes place in a time dependent manner proportional to the amount of protein captured in the wells. Included in this kit for performance of the multiplexing assay are buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.



 
Data
 
Documentation
 
Citations

Product Specifications
 
Species Reactivity: human, bovine
Storage Conditions: Buffers, detergent, substrates, and microplate should be stored at 4°C, Reagent C (reduced Cytochrome c) and Tube 4 should be stored at -20°C.
Country of Origin: USA


Top



Specifications
 
 
Documentation
 
Citations

Figure 1


(click to enlarge)

Figure 1. To determine the activity in the sample, calculate the slope by using microplate software or by manual calculations using one of the two methods shown. Compare the sample rate with the rate of the control (normal) sample and with the rate of the null (background) to get the relative Complex IV activity. (A)The rate is determined by calculating the gradient of the initial slope over the linear region. (B) The rate is determined by calculating the slope between two points within the linear region.

Figure 2


(click to enlarge)

Figure 2. The quantity of Complex IV is expressed as the amount relative to a normal or control sample. Examine the color development and ensure that the rates are linear as shown in the graph. Subtract the initial absorbance reading from the final absorbance reading to determine the relative quantity of Complex IV captured in each well.


Top



Specifications
 
Data
 
 
Citations

Downloadable Documents


   Technical Data Sheet

   Protocol

   Sample Preparation Guide

   MSDS Sodium Azide

   MSDS Diethanolamine


Top



Specifications
 
Data
 
Documentation
 

Published Studies Using This Product: Escobar-Alvarez et al., 2010. Inhibition of Human Peptide Deformylase Disrupts Mitochondrial Function.

Gomez-Duran et al., 2010. Unmasking the causes of multifactorial disorders: OXPHOS differences between mitochondrial haplogroups.

Fogal et al., 2010. Mitochondrial p32 Protein is a Critical Regulator of Tumor Metabolism via Maintenance of Oxidative Phosphorylation.


Have you published studies using this product?
Let us know by filling out this form and receive a $100 discount on your next order.


Top



Browse Products By:
Product Search:


or use our     
Product Finder Tool   


Companion Products:
• Complex IV Human Enzyme Activity
  Dipstick Assay Kit
(cat.#MS430)

• Complex IV FlexPlex™ Module
  (cat.#MSFX-4)

• Complex IV Human Protein Quantity
  Dipstick Assay Kit
(cat.#MS431)

• Complex IV Human Enzyme Activity
  Microplate Assay Kit
(cat.#MS441)



Sales & Customer Support:
1-888-772-2226

[email protected]

© 2004-2011 MitoSciences Inc, an Abcam company. All rights reserved.