Complex II subunit 30 kDa Ip monoclonal antibody
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Catalog No. MS203
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$355.00 - 100 µg
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UniProt Number:
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P21912
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Alternate Names:
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Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, Iron-sulfur subunit of complex II, Ip, SDHB, SDH, SDH1
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Structure and Function:
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This iron-sulfur protein is a component of succinate dehydrogenase. SDH is the subunit that transfers electrons from succinate to coenzyme Q in complex II of the respiratory chain.
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Disease Associations:
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Defects of the iron-sulfur protein of succinate dehydrogenase are associated with pheochromocytoma, hereditary pragangliomas type 4 (PLG4), and Carney-Stratakis syndrome. Pheochromocytoma is a pathology that causes chromaffin tumors in the medulla (90%) or in the extra-adrenal sympathetic ganglia (10%), resulting in hypertension. PLG4 is a pathology that results in the slow development of mainly benign tumors that prefer vascular areas of the head and neck such as the carotid body. Carney-Stratkis syndrome is inherited as a heterozygous dominant condition and is characterized by gastrointestinal stromal tumors and paraganliomas.
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Product Specifications
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Applications:
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Western blotting, Immunocytochemistry (heat-induced antigen-retrieval improves signal), Immunohistochemistry
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Species Reactivity:
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human, bovine, rat, mouse, zebrafish
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Host Species:
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mouse
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Isotype:
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IgG2a, κ
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Clone ID:
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21A11AE7
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Concentration:
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1 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
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Suggested Working Concentration:
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5 µg/mL for Western blotting
5 µg/mL for Immunocytochemistry
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Storage Conditions:
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Store at 4°C. Do not freeze.
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Country of Origin:
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USA
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WB Images
(click to enlarge)
Figure 1. Isolated mitochondria from human heart (lane 1), bovine heart (lane 2), rat heart (lane 3), mouse heart (lane 4), and HepG2 (lane 5) detected with (MS203) anti-30kDa Ip antibody. Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
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ICC Images
(click to enlarge)
Figure 2. Mitochondrial localization of complex II visualized by immunocytochemistry using anti-complex II subunit 30 kDa Ip mAb 21A11AE7 (MS203). Cells were fixed, permeabilized and then labeled with MS204 followed by an Alexa Fluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody.
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IHC Images
(click to enlarge)
Figure 3. Colon Immunohistochemistry – Tissue sections from a normal ageing patient. Kindly provided by Dr. L. Greaves and D. Turnbull, Mitochondrial Research Group, Newcastle University. For more details, see Taylor et al., J. Clin. Invest. 112:1351-1360 (2003).
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Published Studies Using This Product:
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Leveen et al., 2010. The GRACILE mutation introduced into Bcs1l causes postnatal complex III deficiency: A viable mouse model for mitochondrial hepatopathy.
Rayapureddi et al., 2010. TAT-fusion protein transduction into isolated mitochondria is accelerated by sodium channel inhibitors.
Nadanaciva et al., 2010. High-Content Screening for Compounds That Affect mtDNA-Encoded Protein Levels in Eukaryotic Cells.
Kotarsky et al., 2010. Characterization of complex III deficiency and liver dysfunction in GRACILE syndrome caused by a BCS1L mutation.
Fragaki et al., 2009. A neonatal polyvisceral failure linked to a de novo homoplasmic mutation in the mitochondrially encoded cytochrome b gene.
Potluri et al., 2009. A novel NDUFA1 mutation leads to a progressive mitochondrial complex I-specific neurodegenerative disease.
Khidr et al., 2008. Role of SUV3 helicase in maintaining mitochondrial homeostasis in human cells.
Crowe et al., 2008. Metabolic remodeling in adipocytes promotes ciliary neurotrophic factor-mediated fat loss in obesity.
Wijers et al., 2008. Human skeletal muscle mitochondrial uncoupling is associated with cold induced adaptive thermogenesis.
Wilson et al., 2007. Pyruvate induces mitochondrial biogenesis by a PGC-1 alpha-independent mechanism.
Wall et al., 2006. Alterations in oxidative phosphorylation complex proteins in the hearts of transgenic mice that overexpress the p38 MAP kinase activator, MAP kinase kinase 6.
Schrauwen et al., 2006. Reduced skeletal muscle uncoupling protein-3 content in prediabetic subjects and type 2 diabetic patients: restoration by rosiglitazone treatment.
Cisar et al., 2004. Differential expression of mitochondrial electron transport chain proteins in cardiac tissues of broilers from pulmonary hypertension syndrome-resistant and -susceptible lines.
Tomitsuka et al., 2003. Direct evidence for expression of type II flavoprotein subunit in human complex II (succinate-ubiquinone reductase).
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CART
