MitoSciences: MS241 Complex II Enzyme Activity Microplate Assay Kit

Proteins > Complex II > Complex II Enzyme Activity Microplate Assay Kit

Complex II Enzyme Activity Microplate Assay Kit

Catalog No. MS241

$425.00 - 96 tests

Product Description

Kit MS241 is designed for determining the Complex II activity in a human, mouse, rat or bovine sample. Each of the 96 wells in the kit has been coated with an anti-Complex II monoclonal antibody (mAb) which purifies the enzyme from a complex sample such as mitochondria, tissue homogenate or cell lysate. After this in-well purification the production of ubiquinol by the enzyme is coupled to the reduction of the dye DCPIP (2,6-diclorophenolindophenol) and a decreases in its absorbance at 600 nm, which in turn recycles the substrate ubiquinone.

Specifications

Data

Documentation

Citations

Product Specifications
Species Reactivity:	human, rat, mouse, bovine
Storage Conditions:	Store Succinate, Ubiquinone 2 and DCPIP at -80°C. All other components store at 4°C.
Country of Origin:	USA

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Specifications

Data

Documentation

Citations

Figure 1

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Figure 1. Example of raw data. Note the lag period before activity. Also note the activity of mitochondria (BHM, bovine heart mitochondria) is higher than whole cell lysate (HepG2, human hepatoblastoma) and the reaction ends at >1600 seconds because the substrates are used up.

Figure 2

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Figure 2. This assay is compatible with different sample types such as mitochondria, tissue or cell lysates and in multiple species including human and rodent samples. Typical linear range data are shown for MS241.

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