Products for Complex II (succinate dehydrogenase)


- Research Kits
- Individual Antibodies

Overview

Complex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of 4 subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma.

 

Research Kits


MS201 MitoProfile® Complex II Immunocapture Kit
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 µg, ~26 µg or ~39 µg respectively of Complex II from heart mitochondria.

MS202 MitoProfile® Human Complex II Cocktail
Contains a total of 300 µg or 600 µg of antibodies and is a pre-mixed solution of 3 monoclonal antibodies against Complex II subunits 70 kDa Fp, 30 kDa Ip along with an antibody against Complex V α as a control. This cocktail can be used to verify isolated Complex II purity or to conveniently determine the levels of 2 of the 4 Complex II subunits in patient cell lines relative to the control protein (Complex V α).

MS601 MitoProfile® Human Total OXPHOS Complexes Detection Kit
Contains a total of 360 µg or 720 µg of antibodies and is a premixed solution of 5 monoclonal antibodies against a subunit of each of the 5 OXPHOS complexes in mitochondrial preparations from human sources. Human heart mitochondria are also included as a Western blotting control.

MS602 MitoProfile® OXPHOS/PDH Immunocytochemistry Kit
A cocktail of 220 µg antibodies comprised of six mAbs packaged individually. Each antibody is against a labile subunit of each of the 5 OXPHOS complexes and PDH. Each mAb is provided in a 50 µl aliquot at a 200x concentration, to allow reconstitution of each mAb in 10 ml working volume.

MS602a MitoProfile® OXPHOS/PDH Immunocytochemistry Kit
Includes the same components as MS602 (above), but additionally contains 60 µg mAb against porin, and 120 µg against Complex V α subunit, and the required secondary antibodies for simultaneous 2 dye staining of samples.

MS604 MitoProfile® Rodent Total OXPHOS Complexes Detection Kit
Contains a total of 300 µg or 600 µg of antibodies and is a premixed solution of 5 monoclonal antibodies against a subunit of each of the 5 OXPHOS complexes in mitochondrial preparations from rodent sources. Mouse brain mitochondria are also included as a Western blotting control.


 

Individual Antibodies


MS201c Complex II Immunocapture monoclonal antibody
100 µg monoclonal antibody which can immunocapture ~5 g of Complex II from heart mitochondria.

MS203 Complex II subunit 30 kDa Ip monoclonal antibody
100 µg of monoclonal antibody reactive against the Complex II 30 kDa Ip subunit in Western blotting and immunocytochemical applications.

MS204 Complex II subunit 70 kDa Fp monoclonal antibody
100 µg of monoclonal antibody reactive against the Complex II 70 kDa Fp subunit in Western blotting and immunocytochemical applications.

Featured           
Product
MS201 Complex II Immunocapture Kit

This kit allows isolation of the succinate dehydrogenase complex (E.C. 1.3.5.1) from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging.

Uses for the Complex II immunocapture kit include research on aging genetic mitochondrial disease, and various cancers due to mutations in this enzyme complex such as paragangliomas and phaeo-chromocytomas.
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