Complex I subunit NDUFS3 monoclonal antibody

Catalog No. MS112

$325.00 - 100 µg

UniProt Number: O75489
Alternate Names: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, NADH-ubiquinone oxidoreductase 30 kDa subunit, Complex I-30kD, CI-30kD, NDUFS3
Structure and Function: There are 45 unique subunits of Complex I, and this subunit is 264 AA long. It is thought that this subunit is a component of the small assembly in Complex I that is responsible for its catalytic activity.


Product Specifications
Applications: Western blotting
Species Reactivity: human, bovine, rat, mouse, zebrafish, Drosophila, C. elegans (+ weaker 60 kDa band)
Host Species: mouse
Isotype: IgG2a, κ
Clone ID: 17D950C9H11
Concentration: 1 mg/mL in Hepes-Buffered Saline (HBS)-Buffered Saline (HBS) with 0.02% azide as a preservative.
Suggested Working Concentration: 0.5-1 µg/mL for Western blotting
Storage Conditions: Store at 4°C. Do not freeze.
Country of Origin: USA


WB Images

(click to enlarge)

Figure 1. Isolated mitochondria from human heart (lane 1), bovine heart (lane 2), rat heart (lane 3), and mouse heart (lane 4) detected with (MS112) anti-NDUFS3 antibody. Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.



Downloadable Documents

   Technical Data Sheet

   Western blotting Protocol

   MSDS Sodium Azide



Published Studies Using This Product: Suthammarak et al., 2010. Mutations in Mitochondrial Complex III Uniquely Affect Complex I in Caenorhabditis elegans.

Tuppen et al., 2010. The p.M292T NDUFS2 mutation causes complex I-deficient Leigh syndrome in multiple families.

van den Ecker et al., 2010. Blue native electrophoresis to study mitochondrial complex I in C. elegans.

Brys et al., 2010. Disruption of insulin signalling preserves bioenergetic competence of mitochondria in ageing Caenorhabditis elegans.

Shao et al., 2010. Shotgun proteomics analysis of hibernating arctic ground squirrels.

Hoffmann et al., 2009. C. elegans ATAD-3 is essential for mitochondrial activity and development.

Suthammarak et al., 2009. Complex I function is defective in complex IV-deficient Caenorhabditis elegans.

Jiang et al., 2008. Age-associated changes in synaptic lipid raft proteins revealed by two-dimensional fluorescence difference gel electrophoresis.

Calvaruso et al., 2008. Electrophoresis techniques to investigate defects in oxidative phosphorylation.

Ahn et al., 2008. A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis.

Putignani et al., 2008. Alteration of expression levels of the oxidative phosphorylation system (OXPHOS) in breast cancer cell mitochondria.

Rea et al., 2007. Relationship between mitochondrial electron transport chain dysfunction, development, and life extension in Caenorhabditis elegans.

Ventura et al., 2007. Caenorhabditis elegans mitochondrial mutants as an investigative tool to study human neurodegenerative diseases associated with mitochondrial dysfunction.

Callahan et al., 2007. Diaphragm and cardiac mitochondrial creatine kinases are impaired in sepsis.

Busch et al., 2006. Mitochondrial dynamics generate equal distribution but patchwork localization of respiratory Complex I.


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