Complex I subunit NDUFS3 monoclonal antibody

Catalog No. MS110

$325.00 - 100 µg

UniProt Number: O75489
Alternate Names: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, NADH-ubiquinone oxidoreductase 30 kDa subunit, Complex I-30kD, CI-30kD, NDUFS3
Structure and Function: There are 45 unique subunits of Complex I, and this subunit is 264 AA long. It is thought that this subunit is a component of the small assembly in Complex I that is responsible for its catalytic activity.


Product Specifications
Applications: Western blotting, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Species Reactivity: human, bovine, rat, mouse, zebrafish
Host Species: mouse
Isotype: IgG1, κ
Clone ID: 3F9DD2
Concentration: 1 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
Suggested Working Concentration: 0.5 µg/mL for Western blotting
5-10 µg/mL for Immunocytochemistry
1 µg/mL for Flow Cytometry
Storage Conditions: Store at 4°C. Do not freeze.
Country of Origin: USA



WB Images

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Figure 1. Isolated mitochondria from human heart (lane 1), bovine heart (lane 2), rat heart (lane 3), and mouse heart (lane 4) detected with (MS110) anti-NDUFS3 antibody.

ICC Images

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Figure 2. Mitochondrial localization of complex I visualized by immunocytochemistry using anti-complex I subunit NDUFS3 mAb 3F9DD2 (MS110). Cultured human fibroblasts were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with MS110 followed by a fluorescent goat-anti-mouse IgG.

Flow Images

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Figure 3. HeLa cells were stained with 1 µg/mL Complex I subunit NDUFS3 antibody (MS110) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

IHC Images

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Figure 4. Human colon tissue fixed with 4% PFA and antibody detection performed with MS110 using a polymeric labeling two-step method (from Menapath). MS110 primary antibody was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. MS110 diluted 1:100 and incubated for 1 hour. Antigen retrieval was not required.

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Figure 5. Human cerebellum tissue formalin fixed and paraffin-embedded, and detected with MS110 using a polymeric labeling two-step method (from Menapath). MS110 primary antibody was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. MS110 diluted 1:1,000 and incubated for 1 hour. Antigen retrieval performed by 1 min pressure cooking with 1mmol EDTA, pH8.

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Figure 6. Colon Immunohistochemistry – Tissue sections from a normal ageing patient. Kindly provided by Dr. L. Greaves and D. Turnbull, Mitochondrial Research Group, Newcastle University. For more details, see Taylor et al., J. Clin. Invest. 112:1351-1360 (2003).



Downloadable Documents

   Technical Data Sheet

   Western blotting Protocol

   Immunocytochemistry Protocol

   Immunohistochemistry Protocol

   Flow Cytometry Protocol

   MSDS Sodium Azide



Published Studies Using This Product: Ferreira et al., 2011. Progressive cavitating leukoencephalopathy associated with respiratory chain complex I deficiency and a novel mutation in NDUFS1.

Thomas et al., 2010. Recombinant human mitochondrial transcription factor A stimulates mitochondrial biogenesis and ATP synthesis, improves motor function after MPTP, reduces oxidative stress and increases survival after endotoxin.

Fogal et al., 2010. Mitochondrial p32 Protein is a Critical Regulator of Tumor Metabolism via Maintenance of Oxidative Phosphorylation.

Loeb et al., 2010. The transgenic over expression of {alpha}-Synuclein and not its related pathology, associates with complex I inhibition.

Folbergrová et al., 2009. Sustained deficiency of mitochondrial complex I activity during long periods of survival after seizures induced in immature rats by homocysteic acid.

Caro et al., 2009. Effect of 40% restriction of dietary amino acids (except methionine) on mitochondrial oxidative stress and biogenesis, AIF and SIRT1 in rat liver.

Keeney et al., 2009. Mitochondrial gene therapy augments mitochondrial physiology in a Parkinson's disease cell model.

Iyer et al., 2009. Recombinant mitochondrial transcription factor A with N-terminal mitochondrial transduction domain increases respiration and mitochondrial gene expression.

Yang et al., 2009. The effect of different ubiquinones on lifespan in Caenorhabditis elegans.

Murayama et al., 2009. Intractable secretory diarrhea in a Japanese boy with mitochondrial respiratory chain complex I deficiency.

Hakonen et al., 2008. Infantile-onset spinocerebellar ataxia and mitochondrial recessive ataxia syndrome are associated with neuronal complex I defect and mtDNA depletion.

Bastin et al., 2008. Activation of peroxisome proliferator-activated receptor pathway stimulates the mitochondrial respiratory chain and can correct deficiencies in patients' cells lacking its components.

Naudí et al., 2007. Methionine restriction decreases endogenous oxidative molecular damage and increases mitochondrial biogenesis and uncoupling protein 4 in rat brain.

Lebon et al., 2007. A novel mutation of the NDUFS7 gene leads to activation of a cryptic exon and impaired assembly of mitochondrial complex I in a patient with Leigh syndrome.

Fosset et al., 2006. RNA silencing of mitochondrial m-Nfs1 reduces Fe-S enzyme activity both in mitochondria and cytosol of mammalian cells.

Grad et al., 2006. Riboflavin enhances the assembly of mitochondrial cytochrome c oxidase in C. elegans NADH-ubiquinone oxidoreductase mutants.

Schilling et al., 2005. Rapid purification and mass spectrometric characterization of mitochondrial NADH dehydrogenase (Complex I) from rodent brain and a dopaminergic neuronal cell line.


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