ATP synthase subunit alpha monoclonal antibody
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Catalog No. MS502
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$325.00 - 100 µg
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UniProt Number:
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P25705
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Alternate Names:
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Complex V subunit alpha, ATP5A1, ATP5A, ATP5AL2, ATPM
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Structure and Function:
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ATPsynthase (complex V) is responsible for catalyzing the formation of ATP from ADP and Pi using energy from the proton concentration gradient created by preceding complexes of the respiratory chain. The two main components of this complex are F1 and F0, which are responsible for catalysis and transmembrane proton transport respectively. The alpha and beta subunits make up the catalytic core (F1) of ATPsynthase.
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Product Specifications
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Applications:
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Western blotting, Immunocytochemistry, Flow Cytometry
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Species Reactivity:
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human, bovine, mouse, rat, zebrafish
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Host Species:
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mouse
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Isotype:
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IgG2b, κ
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Clone ID:
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7H10BD4F9
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Concentration:
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1 mg/mL in Hepes -Buffered Saline (HBS) with 0.02% azide as a preservative.
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Suggested Working Concentration:
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1-2 µg/mL for Western blotting
5 µg/mL for Immunocytochemistry
1 µg/mL for Flow Cytometry
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Storage Conditions:
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Store at 4°C. Do not freeze.
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Country of Origin:
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USA
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WB Images
(click to enlarge)
Figure 1. Isolated mitochondria from human heart (lane 1), bovine heart (lane 2), rat heart (lane 3), mouse heart (lane 4), and HepG2 (lane 5) detected with (MS502) anti-alpha antibody.
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ICC Images
(click to enlarge)
Figure 2. Mitochondrial localization of ATP synthase visualized by immunocytochemistry using anti-ATP synthase subunit alpha mAb 7H10BD4F9 (MS502). Cultured human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with MS502 (2 µg/ml) followed by an Alexa Fluor® 594-conjugated-goat-anti-mouse IgG2b isotype specific secondary antibody (2 µg/ml).
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Flow Images
(click to enlarge)
Figure 3. HL-60 cells were stained with 1 µg/mL ATP synthase subunit alpha antibody (MS502) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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Published Studies Using This Product:
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Mayr et al., 2010. Mitochondrial ATP synthase deficiency due to a mutation in the ATP5E gene for the F1 {varepsilon} subunit.
Song et al., 2010. Mice deficient in group VIB phospholipase A2 (iPLA2gamma) exhibit relative resistance to obesity and metabolic abnormalities induced by a Western diet.
Perocchi et al., 2010. MICU1 encodes a mitochondrial EF hand protein required for Ca2+ uptake
Fogal et al., 2010. Mitochondrial p32 Protein is a Critical Regulator of Tumor Metabolism via Maintenance of Oxidative Phosphorylation.
Havlickova et al., 2009. Knockdown of F(1) epsilon subunit decreases mitochondrial content of ATP synthase and leads to accumulation of subunit c.
Uittenbogaard et al., 2009. Cloning and characterization of the 5'UTR of the rat anti-apoptotic Bcl-w gene.
Ogawa et al., 2009. Heterogeneous levels of oxidative phosphorylation enzymes in rat adrenal glands.
Houstek et al., 2009. TMEM70 protein - a novel ancillary factor of mammalian ATP synthase.
Song et al., 2009. Analysis of protein expression and compartmentalization in retinal neurons using serial tangential sectioning of the retina.
Calvaruso et al., 2008. Electrophoresis techniques to investigate defects in oxidative phosphorylation.
Nakamura et al., 2008. Optical reporters for the conformation of alpha-synuclein reveal a specific interaction with mitochondria.
Nordgaard et al., 2008. Mitochondrial proteomics of the retinal pigment epithelium at progressive stages of age-related macular degeneration.
Son et al., 2008. Isolated cytochrome c oxidase deficiency in G93A SOD1 mice overexpressing CCS protein.
Mitsuishi et al., 2008. cGMP rescues mitochondrial dysfunction induced by glucose and insulin in myocytes.
Kim et al., 2008. Increased expression of the F(1)F(o) ATP synthase in response to iron in heart mitochondria.
Zheng et al., 2008. The C-terminal transmembrane domain of Bcl-xL mediates changes in mitochondrial morphology.
Margineantu et al., 2007. Hsp90 inhibition decreases mitochondrial protein turnover.
Csiszar et al., 2007. Vascular aging in the longest-living rodent, the naked mole rat.
Liu et al., 2007. U bodies are cytoplasmic structures that contain uridine-rich small nuclear ribonucleoproteins and associate with P bodies.
Deng et al., 2006. Nuclear suppression of mitochondrial defects in cells without the ND6 subunit.
Stenzinger et al., 2006. Epidermal growth factor-, transforming growth factor-beta-, retinoic acid- and 1,25-dihydroxyvitamin D3-regulated expression of the novel protein PTPIP51 in keratinocytes.
Graham et al., 2005. Functional organization of the bovine rumen epithelium.
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