Alterations in the ratio of bax and bcl-2 like proteins regulate the permeability of the mitochondrial outer membrane and can result in the release of several proteins from the inter-membrane space, including cytochrome c, SMAC-Diablo and AIF (apoptosis-inducing factor). Cytochrome c then reacts with a cytosolic protein APAF to induce an irreversible cascade of events involving caspases that lead to the orderly degradation of proteins and DNA.
A key contributor to the altered permeability of the organelle in apoptosis is the so-called permeability transition pore (PTP). This complex remains to be fully characterized but is thought to include hexokinase, porin and the peripheral benzodiazepine receptor of the outer membrane, adenylate kinase of the intermembrane space, ANT (the adenine nucleotide transporter) of the inner membrane and cyclophilin D from the matrix space. MitoSciences has mAbs to several of the PTP proteins.
Monoclonal Antibodies
Cat. No. | Name | Reactivity | Apps. | Amount | Price | |
MSA09 | AIF antibody | human | WB, ICC, ICE, IP | 100 µg | $325.00 | |
MS105 |
Complex I subunit NDUFB8 |
human, mouse, rat, bovine | WB, BNPAGE | 100 µg | $325.00 | |
MS110 |
Complex I subunit NDUFS3 |
human, mouse, rat, bovine, zebrafish | WB, ICC, IHC | 100 µg | $325.00 | |
MS112 |
Complex I subunit NDUFS3 |
human, mouse, rat, bovine, zebrafish, Drosophila, C. elegans | WB | 100 µg | $325.00 | |
MSA06 | Cytochrome c antibody | human, bovine, rat, mouse, C. elegans | WB, ICC, ICE | 100 µg | $325.00 | |
MS777 | PARP-1 antibody | human | WB, ICC, ICE | 100 µg | $325.00 | |
MS715 | SMAC antibody | human | ICC | 100 µg | $325.00 | |
MS719 | SMAC antibody | human | WB | 100 µg | $325.00 |
Antibody Cocktails & Kits
Cat. No. | Name | Reactivity | Amount | Price | |
MSA12 |
Cytochrome c Apoptosis Antibody Cocktail |
human | 180 µg | $425.00 | |
MSA07 |
Cytochrome c Apoptosis ICC Antibody Kit |
human, bovine, rat, mouse, C. elegans | 200 µg | $425.00 |
Sample Prep
Cat. No. | Name | Price | |
MS861 | Cell Fractionation Kit - Standard | $195.00 |
Protein Quantity Assays
Cat. No. | Name | Reactivity | Amount | Price | |
MSFX-18 | AIF FlexPlex™ Module | human | 4 strips (32 tests) | $125.00 | |
MSFX-1 | Complex I FlexPlex™ Module | human, bovine | 4 strips (32 tests) | $125.00 | |
MSFX-16 | Cytochrome c FlexPlex™ Module | human, bovine | 4 strips (32 tests) | $125.00 | |
MSA41 | Cytochrome c Protein Quantity Microplate Assay Kit | human, bovine, mouse, rat | 96 tests | $425.00 |
In-Cell ELISA Kits
Cat. No. | Name | Reactivity | Amount | Price | |
MSA43 | PARP-1 (cleaved) In-Cell ELISA Kit | human | 2 x 96 tests | $425.00 |