Mitochondria are at the center of apoptosis, also known as programmed cell death. Intra-cellular and extra-cellular signals alter the association of a set of cytosolic pro-apoptotic and anti-apoptotic proteins with the organelle. These include the bax and bcl-2 families of proteins.

Alterations in the ratio of bax and bcl-2 like proteins regulate the permeability of the mitochondrial outer membrane and can result in the release of several proteins from the inter-membrane space, including cytochrome c, SMAC-Diablo and AIF (apoptosis-inducing factor). Cytochrome c then reacts with a cytosolic protein APAF to induce an irreversible cascade of events involving caspases that lead to the orderly degradation of proteins and DNA.

A key contributor to the altered permeability of the organelle in apoptosis is the so-called permeability transition pore (PTP). This complex remains to be fully characterized but is thought to include hexokinase, porin and the peripheral benzodiazepine receptor of the outer membrane, adenylate kinase of the intermembrane space, ANT (the adenine nucleotide transporter) of the inner membrane and cyclophilin D from the matrix space. MitoSciences has mAbs to several of the PTP proteins.

Monoclonal Antibodies
Cat. No. Name Reactivity Apps. Amount Price  
MSA09 AIF antibody human WB, ICC, ICE, IP 100 µg $325.00
MS105 Complex I
subunit NDUFB8
human, mouse, rat, bovine WB, BNPAGE 100 µg $325.00
MS110 Complex I
subunit NDUFS3
human, mouse, rat, bovine, zebrafish WB, ICC, IHC 100 µg $325.00
MS112 Complex I
subunit NDUFS3
human, mouse, rat, bovine, zebrafish, Drosophila, C. elegans WB 100 µg $325.00
MSA06 Cytochrome c antibody human, bovine, rat, mouse, C. elegans WB, ICC, ICE 100 µg $325.00
MS777 PARP-1 antibody human WB, ICC, ICE 100 µg $325.00
MS715 SMAC antibody human ICC 100 µg $325.00
MS719 SMAC antibody human WB 100 µg $325.00

Antibody Cocktails & Kits
Cat. No. Name Reactivity Amount Price  
MSA12 Cytochrome c Apoptosis
Antibody Cocktail
human 180 µg $425.00
MSA07 Cytochrome c Apoptosis
ICC Antibody Kit
human, bovine, rat, mouse, C. elegans 200 µg $425.00

Sample Prep
Cat. No. Name Price  
MS861 Cell Fractionation Kit - Standard $195.00

Protein Quantity Assays
Cat. No. Name Reactivity Amount Price  
MSFX-18 AIF FlexPlex™ Module human 4 strips (32 tests) $125.00
MSFX-1 Complex I FlexPlex™ Module human, bovine 4 strips (32 tests) $125.00
MSFX-16 Cytochrome c FlexPlex™ Module human, bovine 4 strips (32 tests) $125.00
MSA41 Cytochrome c Protein Quantity Microplate Assay Kit human, bovine, mouse, rat 96 tests $425.00

In-Cell ELISA Kits
Cat. No. Name Reactivity Amount Price  
MSA43 PARP-1 (cleaved) In-Cell ELISA Kit human 2 x 96 tests $425.00

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