This kit is used to quantitate the amount of AIF in a human sample. It uses the well-established sandwich ELISA format, whereby a pair of monoclonal antibodies, each against a different epitope, is used to capture and then detect the amount of protein in a sample.
AIF is immunocaptured within the wells of a pre-coated microplate and the quantity of enzyme is measured by adding a second AIF-specific antibody that is labeled with horseradish peroxidase. This peroxidase changes the substrate from colorless to blue and the rate of color development is proportional to the amount of protein captured in the well (monitored at 600 nm). Alternatively the assay can be terminated, at a user-defined time, by the addition of 1N HCl (not supplied) and the assay performed as an end point measurement at 450 nm.