Apoptosis-Inducing Factor (AIF) monoclonal antibody
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Catalog No. MSA09
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$325.00 - 100 µg
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Product Specifications
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Applications:
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Western blotting, Immunocytochemistry, Immunoprecipitation, In-Cell ELISA, Flow Cytometry
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Species Reactivity:
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human
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Host Species:
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mouse
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Isotype:
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IgG1, κ
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Clone ID:
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7F7AB10
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Concentration:
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1 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
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Suggested Working Concentration:
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2-5 µg/mL for Western blotting
5-10 µg/mL for Immunocytochemistry
1 µg/mL for In-Cell ELISA (0.1 µg/well)
1 µg/mL for Flow Cytometry
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Storage Conditions:
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Store at 4°C. Do not freeze.
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Country of Origin:
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USA
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WB Images
(click to enlarge)
Figure 1. Isolated mitochondria from human heart detected with (MSA09) anti-AIF antibody.
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ICC Images
(click to enlarge)
Figure 2. Immunocytochemistry image of MSA09 stained human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the antibody (7F7AB10, 5 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
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Flow Images
(click to enlarge)
Figure 3. HL-60 cells were stained with 1 µg/mL AIF antibody (MSA09) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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