MS103 Complex I subunit GRIM-19 monoclonal antibody
100 µg of monoclonal antibody reactive against the 19 kDa Complex I (NADH dehydrogenase) subunit GRIM-19. MS103 also labels GRIM-19 in immunocytochemical applications. Interestingly GRIM-19 is also present in the nucleus and cytoplasm. Its expression is necessary for interferonβ/retinoic acid induced tumor mortality.
MS107 Complex I subunit NDUFB4 monoclonal antibody
100 µg of monoclonal antibody reactive against the 15 kDa Complex I (NADH dehydrogenase) subunit NDUFB4. MS107 also labels in immunocytochemical applications. Formerly listed as likely subunit NDUFA6.
MS404 Complex IV subunit I monoclonal antibody
100 µg of monoclonal antibody reactive against the 57 kDa COX (Complex IV) subunit I. The apparent molecular weight is 35 kDa. MS404 also labels COX I in immunocytochemical applications.
MS506 ATP synthase (Complex V) subunit IF1 monoclonal antibody
100 µg of monoclonal antibody reactive against the ATP synthase (Complex V) IF1 subunit. The antibody shows in Western blotting of whole mitochondria reactivity with two bands at 10 kDa and at 18 kDa, but in isolated F1F0 only the band at 10 kDa is observed (IF1). The 18 kDa band is the subunit IV of Complex IV.
MS701 NNT Monoclonal Antibody
Nicotinamide nucleotide transhydrogenase (NNT) is a mitochondrial inner membrane protein catalyzing the interconversion of NADH and NADPH within the matrix space of the organelle. It couples this reaction to proton translocation across the inner membrane. In the presence of an electrochemical proton gradient the production of NADPH is strongly favored. NADPH is required in the mitochondrial matrix to keep the ratio GSH:GSSG high via the NADPH dependent glutathione reductase. Thus NNT has an important role to play in defences against oxidative stress produced by the organelle. A novel, unexpected role of NNT is in insulin secretion. The absence of NNT prevents glucose from closing the K+ATP channel and from opening the Ca++ channel in pancreatic beta cells. These defective beta cells also show a profound increase inROS.
MSM02 Mitofilin monoclonal antibody
100 µg of monoclonal antibody against mitofilin, suitable for use in Western Blotting and Immunocytochemistry. The mitofilin monoclonal antibody reacts against 2 isoforms (about 90 and 91kDa) in Western blots with human heart mitochondria. A single form of mitofilin is detected in Western blots with human liver mitochondria.
MSP06 PDH subunit E2/E3bp monoclonal antibody
100 µg of monoclonal antibody reactive against both the 66 kDa PDH E2 subunit and the 54 kDa E3 binding protein. Preliminary data suggest that this antibody recognizes bound lipoic acid. MSP06 also labels E2 & E3bp in immunocytochemical applications.