IntroductionTwo species of yeast have been pressed into service as model organisms: S. cerevisiae and S. pombe, largely because the cell cycle in a yeast cell is very similar to the cell cycle in humans, and therefore the basic cellular mechanics of DNA replication, recombination, cell division and metabolism are comparable. Also, yeasts are easily manipulated and cultured in the lab which has allowed for the development of powerful standard techniques. Many proteins important in human biology were first discovered by studying their homologs in yeast, including cell cycle proteins, signaling proteins, and protein-processing enzymes.
Researchers working with S. cerevisiae are often challenged to find high quality antibodies to relevant targets of interest. Scientists at MitoSciences have increased their efforts to validate high quality anti-yeast antibodies against targets in mitochondria, vacuoles and other cellular targets of interest. Antibodies are currently characterized for reactivity in Western blot (WB) with yeast cell and membrane lysate samples and many are also suitable for immunocytochemistry (ICC).