Western Blotting Mitochondrial Antibodies

Products for Western Blotting


- Research Kits
- Individual Antibodies

Overview

MitoSciences provides individual antibodies as well as pre-mixed cocktails for the detection of mitochondrial proteins by Western blotting. These antibodies bind various subunits of OXPHOS complexes I, II, III, IV and V in addition to other proteins of the inner mitochondrial membrane. A series of antibodies are also available against subunits of the pyruvate dehydrogenase complex (PDH).

 

Research Kits


MS601 MitoProfile® Human Total OXPHOS Complexes Detection Kit
Contains a total of 360 g or 720 g of antibodies and is a premixed solution of 5 monoclonal antibodies against a subunit of each of the 5 OXPHOS complexes. Human heart mitochondria are also included as a Western blotting control.

MS604 MitoProfile® Rodent Total OXPHOS Complexes Detection Kit
Contains a total of 300 µg or 600 µg of antibodies and is a premixed solution of 5 monoclonal antibodies against a subunit of each of the 5 OXPHOS complexes in mitochondrial preparations from rodent sources. Mouse brain mitochondria are also included as a Western blotting control.

MSA12 MitoProfile® Apoptosis Detection Kit for Western blotting
Contains 180 µg of premixed cocktail of 4 monoclonal antibodies that allows detection of cytochrome c in the cytoplasmic and mitochondria-containing fractions by Western Blot analysis to determine the proportion of released cytochrome c from mitochondria to cytoplasm. Human heart mitochondria are also included as a Western blotting control.

 

Individual Antibodies


MS103 Complex I subunit GRIM-19 monoclonal antibody
100 µg of monoclonal antibody reactive against the 19 kDa Complex I (NADH dehydrogenase) subunit GRIM-19. MS103 also labels GRIM-19 in immunocytochemical applications. Interestingly GRIM-19 is also present in the nucleus and cytoplasm. Its expression is necessary for interferonβ/retinoic acid induced tumor mortality.

MS104 Complex I subunit NDUFS4 monoclonal antibody
100 µg of monoclonal antibody reactive against the 18 kDa Complex I (NADH dehydrogenase) subunit NDUFS4.

MS105 Complex I subunit NDUFB8 monoclonal antibody
100 µg of monoclonal antibody reactive against the 20kDa Complex I (NADH dehydrogenase) subunit NDUFB8. Formerly listed as likely subunit ND6.

MS107 Complex I subunit NDUFB4 monoclonal antibody
100 µg of monoclonal antibody reactive against the 15 kDa Complex I (NADH dehydrogenase) subunit NDUFB4. MS107 also labels in immunocytochemical applications. Formerly listed as likely subunit NDUFA6.

MS108 Complex I subunit NDUFB6 monoclonal antibody
100 µg of monoclonal antibody reactive against the 17 kDa Complex I (NADH dehydrogenase) subunit NDUFB6.

MS109 Complex I subunit 8 kDa monoclonal antibody kDa
100 µg of monoclonal antibody reactive against an 8 kDa Complex I (NADH dehydrogenase) subunit.

MS110 Complex I subunit NDUFS3 monoclonal antibody
100 µg of monoclonal antibody reactive against the 30 kDa Complex I (NADH dehydrogenase) subunit NDUFS3. MS110 also labels NDUFS3 in immunocytochemical applications.

MS111 Complex I subunit NDUFA9 monoclonal antibody
100 µg of monoclonal antibody reactive against the 39 kDa Complex I (NADH dehydrogenase) subunit NDUFA9. Human heart mitochondria are also included as a Western blotting control.

MS112 Complex I subunit NDUFS3 monoclonal antibody
100 µg of monoclonal antibody reactive against the 30 kDa Complex I (NADH dehydrogenase) subunit NDUFS3 in humans and C. elegans.

MS202 Human Complex II Cocktail
Contains a total of 300 µg or 600 µg of antibodies and is a pre-mixed solution of 3 monoclonal antibodies against Complex II subunits 70 kDa Fp, 30 kDa Ip along with an antibody against Complex V α as a control. This cocktail can be used to verify isolated Complex II purity or to conveniently determine the levels of 2 of the 4 Complex II subunits in patient cell lines relative to the control protein (Complex V α).

MS203 Complex II subunit 30 kDa Ip monoclonal antibody
100 µg of monoclonal antibody reactive against the Complex II 30 kDa Ip subunit in Western blotting and immunocytochemical applications.

MS204 Complex II subunit 70 kDa Fp monoclonal antibody
100 µg of monoclonal antibody reactive against the Complex II 70 kDa Fp subunit in Western blotting and immunocytochemical applications.

MS303 Complex III subunit Core 1 monoclonal antibody
100 µg of monoclonal antibody reactive against the 49 kDa complex III Core protein 1.

MS304 Complex III subunit Core 2 monoclonal antibody
100 µg of monoclonal antibody reactive against the 47 kDa Complex III Core protein 2. MS304 also labels core 2 in immunocytochemical applications.

MS305 Complex III subunit Rieske monoclonal antibody
100 µg of monoclonal antibody reactive against the 22 kDa complex III Rieske subunit.

MS306 Complex III subunit 10 kDa monoclonal antibody
100 µg of monoclonal antibody reactive against the complex III 10 kDa subunit.

MS403 Complex IV subunit SURF1 monoclonal antibody
100 µg of monoclonal antibody reactive against the 33 kDa Complex IV assembly factor SURF1. Cellular levels of SURF1 are highest in growing cultured cells.

MS404 Complex IV subunit I monoclonal antibody
100 µg of monoclonal antibody reactive against the 57 kDa COX (Complex IV) subunit I. The apparent molecular weight is 35 kDa. MS404 also labels COX I in immunocytochemical applications.

MS405 Complex IV subunit II monoclonal antibody
100 µg of monoclonal antibody reactive against the 26 kDa COX (Complex IV) subunit II. MS405 also labels COX II in immunocytochemical applications.

MS406 Complex IV subunit III monoclonal antibody
100 µg of monoclonal antibody reactive against the 30 kDa COX (Complex IV) subunit III. The apparant molecular weight is 22 kDa.

MS407 Complex IV subunit IV monoclonal antibody
100 µg of monoclonal antibody reactive against the 18 kDa COX (Complex IV) subunit IV. Reacts with human, bovine, mouse and rat. MS408 is an alternative antibody against COX IV.

MS408 Complex IV subunit IV monoclonal antibody (alternate to MS407)
100 µg of monoclonal antibody reactive against the 18 kDa COX (Complex IV) subunit IV. MS408 also labels COX IV in immunocytochemical applications. Reacts only with human and bovine, but better than MS407 for immunocytochemistry in these species.

MS409 Complex IV subunit Va monoclonal antibody
100 µg of monoclonal antibody reactive against the 12 kDa COX (Complex IV) subunit Va. MS409 also labels COX Va in immunocytochemical applications.

MS410 Complex IV subunit Vb monoclonal antibody
100 µg of monoclonal antibody reactive against the 13 kDa COX (Complex IV) subunit Vb.

MS411 Complex IV subunit VIaH monoclonal antibody
250 µg of monoclonal antibody reactive against the 12 kDa COX (Complex IV) subunit VIa and specific to the 'heart' isoform.

MS412 Complex IV subunit VIaL monoclonal antibody
250 µg of monoclonal antibody reactive against the 12 kDa COX (Complex IV) subunit VIa and specific to the 'liver' isoform.

MS413 Complex IV subunit VIb monoclonal antibody
100 µg of monoclonal antibody reactive against the 12 kDa COX (Complex IV) subunit VIb.

MS414 Complex IV subunit VIc monoclonal antibody
100 µg of monoclonal antibody reactive against the 12 kDa COX (Complex IV) subunit VIc.

MS415 Complex IV subunit VIIaHL monoclonal antibody
250 µg of monoclonal antibody reactive against the 8 kDa COX (Complex IV) subunit VIIa and reacting against both 'liver' and 'heart' isoforms.

MS416 Complex IV subunit VIIb monoclonal antibody
250 µg of monoclonal antibody reactive against the 8 kDa COX (Complex IV) subunit VIIb.

MS418 Complex IV subunit I monoclonal antibody for yeast
100 µg of monoclonal antibody reactive against the COX (Complex IV) subunit I in Saccharomyces cerevisiae for use in Western blotting applications.

MS419 Complex IV subunit II monoclonal antibody for yeast
100 µg of monoclonal antibody reactive against the COX (Complex IV) subunit II in Saccharomyces cerevisiae for use in Western blotting applications.

MS420 Complex IV subunit IV monoclonal antibody for yeast
100 µg of monoclonal antibody reactive against the COX (Complex IV) subunit IV in Saccharomyces cerevisiae for use in Western blotting applications.

MS502 ATP synthase (Complex V) subunit α monoclonal antibody
100 µg of monoclonal antibody reactive against the 55 kDa F1F0 ATP synthase (Complex V) α subunit. MS502 also labels α in immunocytochemical applications.

MS503 ATP synthase (Complex V) subunit β monoclonal antibody
100 µg of monoclonal antibody reactive against 52 kDa the F1F0 ATP synthase (Complex V) β subunit. MS503 also labels β in immunocytochemical applications.

MS504 ATP synthase (Complex V) subunit d monoclonal antibody
100 µg of monoclonal antibody reactive against the 19 kDa F1F0 ATP synthase (Complex V) d subunit. MS504 also labels the d subunit in immunocytochemical applications.

MS505 ATP synthase (Complex V) subunit OSCP monoclonal antibody
100 µg of monoclonal antibody reactive against the 21 kDa F1F0 ATP synthase (Complex V) OSCP subunit. MS505 also labels the OSCP subunit in immunocytochemical applications.

MS506 ATP synthase (Complex V) subunit IF1 monoclonal antibody
100 µg of monoclonal antibody reactive against the Complex V IF1 subunit. The antibody shows in Western blotting of whole mitochondria reactivity with two bands at 10 kDa and at 18 kDa, but in isolated F1F0 only the band at 10 kDa is observed (IF1). The 18 kDa band is the subunit IV of Complex IV.

MS507 ATP synthase (Complex V) subunit α monoclonal antibody with broad cross-reactivity
100 µg of monoclonal antibody reactive against the 55 kDa F1F0 ATP synthase (Complex V) α subunit. This mAb has broad species cross-reactivity including animal and plant subunits. MS507 also labels the α subunit in immunocytochemical applications.

MS508 ATP synthase (Complex V) subunit F6 monoclonal antibody
100 µg of monoclonal antibody reactive against the 9 kDa F1F0 ATP synthase (Complex V) F6 subunit. MS508 also labels the F6 subunit in immunocytochemical applications.

MS511 ATP synthase (Complex V) subunit anti-F1F0-beta monoclonal antibody
100 µg of monoclonal antibody reactive against the 9 kDa F1F0 ATP synthase (Complex V) beta subunit.

MS701 NNT Monoclonal Antibody
Nicotinamide nucleotide transhydrogenase (NNT) is a mitochondrial inner membrane protein catalyzing the interconversion of NADH and NADPH within the matrix space of the organelle. It couples this reaction to proton translocation across the inner membrane. In the presence of an electrochemical proton gradient the production of NADPH is strongly favored. NADPH is required in the mitochondrial matrix to keep the ratio GSH:GSSG high via the NADPH dependent glutathione reductase. Thus NNT has an important role to play in defences against oxidative stress produced by the organelle. A novel, unexpected role of NNT is in insulin secretion. The absence of NNT prevents glucose from closing the K+ATP channel and from opening the Ca++ channel in pancreatic beta cells. These defective beta cells also show a profound increase inROS.

MS702 Mitochondrial Trifunctional Protein (TFP) Monoclonal Antibody
100 ug of monoclonal antibody reactive against the mitochondrial trifunctional protein (TFP).

MSA02 ANT monoclonal antibody
100 µg of monoclonal antibody reactive against the 32 kDa adenine nucleotide translocase (ANT). MSA02 also labels ANT in immunocytochemical applications.

MSA03 Porin monoclonal antibody
100 µg of monoclonal antibody reactive against the 39 kDa human porin (VDAC) for Western blotting applications.

MSA04 Cyclophilin D monoclonal antibody
100 µg of monoclonal antibody reactive against the 21 kDa human Cyclophilin 'D' (P30405). MSA04 also labels Cyclophilin D in immunocytochemical applications.

MSA06 Cytochrome c monoclonal antibody
100 µg of monoclonal antibody against the 12 kDa Cytochrome c, suitable for use in Western Blotting and Immunocytochemistry.

MSA08 Porin monoclonal antibody for yeast
100 µg of monoclonal antibody reactive against Porin in Saccharomyces cerevisiae for use in Western blotting applications.

MSA09 AIF (Apoptosis-Inducing Factor) monoclonal antibody
100 µg of monoclonal antibody reactive against the 67 kDa AIF, suitable for use in for Western blotting and immunocytochemistry applications.

MSF01 Frataxin monoclonal antibody
100 µg of monoclonal antibody against frataxin, suitable for use in Western Blotting and Immunocytochemistry.

MSM02 Mitofilin monoclonal antibody
100 µg of monoclonal antibody against mitofilin, suitable for use in Western Blotting and Immunocytochemistry. Human heart mitochondria are also included as a Western Blotting control. The mitofilin monoclonal antibody reacts against 2 isoforms (about 90 and 91kDa) in Western blots with human heart mitochondria. A single form of mitofilin is detected in Western blots with human liver mitochondria.

MSP03 PDH subunit E1α monoclonal antibody
100 g of monoclonal antibody reactive against the 44 kDa PDH E1α subunit. MSP03 also labels E1α in immunocytochemical applications

MSP04 PDH subunit E1β monoclonal antibody
100 g of monoclonal antibody reactive against the 39 kDa PDH subunit E1β.

MSP05 PDH subunit E2 monoclonal antibody
100 g of monoclonal antibody reactive against the 66 kDa PDH E2 subunit. MSP05 also labels E2 in immunocytochemical applications.

MSP06 PDH subunit E2/E3bp monoclonal antibody
100 g of monoclonal antibody reactive against both the 66 kDa PDH E2 subunit and the 54 kDa E3 binding protein. Preliminary data suggest that this antibody recognizes bound lipoic acid. MSP06 also labels E2 & E3bp in immunocytochemical applications.

MSP07 PDH subunit E1α monoclonal antibody
100 g of monoclonal antibody reactive against the 44 kDa PDH E1α subunit. MSP07 also labels E1α in immunocytochemical applications.


Featured           
Product
MS601 MitoProfile® Human Total OXPHOS Complexes Detection Kit

The kit is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from human sources.
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