Pyruvate dehydrogenase (PDH) subunit E2 monoclonal antibody

Catalog No. MSP05

$325.00 - 100 µg

UniProt Number: P10515
Alternate Names: Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, Pyruvate dehydrogenase complex component E2, PDC-E2, PDCE2, 70 kDa mitochondrial autoantigen of primary biliary cirrhosis, PBC, M2 antigen complex 70 kDa subunit, DLAT, DLTA
Structure and Function: The pyruvate dehydrogenase complex contains three different subunits including the dihydrolipoamide acetyltransferase (E2) subunit.
Disease Associations: The E2 subunit of the PHD complex is associated with primary biliary cirrhosis in that patients diagnosed with this disease have autoantibodies for this subunit. This condition causes damage to liver cells and can result in cirrhosis of the liver. Lactic acidemia (PDH deficiency) is another condition associated with the E2 subunit. The clinical characterization of this disorder includes primary lactic acidosis as well as neurological problems, mainly episodic dystonia.


Product Specifications
Applications: Western blotting, Immunohistochemistry, Immunocytochemistry (heat-induced antigen-retrieval improves signal), In-Cell ELISA, Flow Cytometry
Species Reactivity: human, bovine
Host Species: mouse
Isotype: IgG1, κ
Clone ID: 15D3G9C11
Concentration: 1.0 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
Suggested Working Concentration: 0.5 µg/mL for Western blotting
0.2-0.5 µg/mL for Immunocytochemistry
1 µg/mL for In-Cell ELISA (0.1 µg/well)
1 µg/mL for Flow Cytometry
Storage Conditions: Store at 4°C. Do not freeze.
Country of Origin: USA


WB Images

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Figure 1. Isolated mitochondria from human heart detected with (MSP05) anti-subunit E2 antibody.
ICC Images

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Figure 2. Mitochondrial localization of PDH in cultured, normal human embryonic lung fibroblasts (strain MRC5) labeled with anti-PDH-E2 mAb 15D3G9C11 (MSP05) as the primary antibody (1 ug/ml) and an AlexaFluor® 488 goat anti-mouse IgG1 secondary antibody (2 ug/ml).
IHC Images

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Figure 3. PDH subunit E2 immunohistochemistry in human cerebellum visualized with mAb MSP05. PDH subunit E2 immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells. Image kindly provided by Nichola Lax and Doug Turnbull, Mitochondrial Research Group, Newcastle University.
Flow Images

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Figure 4. HL-60 cells were stained with 1 µg/mL PDH subunit E2 antibody (MSP05) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.



Downloadable Documents

   Technical Data Sheet

   Western blotting Protocol

   Immunocytochemistry Protocol

   Immunohistochemistry Protocol

   In-Cell ELISA Protocol

   Flow Cytometry Protocol

   PDH Playbook

   MSDS Sodium Azide



Published Studies Using This Product: Feng et al., 2009. Down-regulation of mitochondrial acyl carrier protein in mammalian cells compromises protein lipoylation and respiratory complex I and results in cell death.

Ridout et al., 2008. Somatic mosaicism for a PDHA1 mutation in a female with pyruvate dehydrogenase deficiency.

Han et al., 2008. A combined therapeutic approach for pyruvate dehydrogenase deficiency using self-complementary adeno-associated virus serotype-specific vectors and dichloroacetate.

Stiburek et al., 2007. Knockdown of human Oxa1l impairs the biogenesis of F1Fo-ATP synthase and NADH:ubiquinone oxidoreductase.

Koukourakis et al., 2005. Pyruvate dehydrogenase and pyruvate dehydrogenase kinase expression in non small cell lung cancer and tumor-associated stroma.


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