Pyruvate dehydrogenase (PDH) subunit E2 monoclonal antibody
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Catalog No. MSP05
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$325.00 - 100 µg
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UniProt Number:
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P10515
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Alternate Names:
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Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, Pyruvate dehydrogenase complex component E2, PDC-E2, PDCE2, 70 kDa mitochondrial autoantigen of primary biliary cirrhosis, PBC, M2 antigen complex 70 kDa subunit, DLAT, DLTA
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Structure and Function:
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The pyruvate dehydrogenase complex contains three different subunits including the dihydrolipoamide acetyltransferase (E2) subunit.
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Disease Associations:
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The E2 subunit of the PHD complex is associated with primary biliary cirrhosis in that patients diagnosed with this disease have autoantibodies for this subunit. This condition causes damage to liver cells and can result in cirrhosis of the liver. Lactic acidemia (PDH deficiency) is another condition associated with the E2 subunit. The clinical characterization of this disorder includes primary lactic acidosis as well as neurological problems, mainly episodic dystonia.
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Product Specifications
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Applications:
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Western blotting, Immunohistochemistry, Immunocytochemistry (heat-induced antigen-retrieval improves signal), In-Cell ELISA, Flow Cytometry
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Species Reactivity:
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human, bovine
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Host Species:
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mouse
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Isotype:
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IgG1, κ
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Clone ID:
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15D3G9C11
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Concentration:
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1.0 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
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Suggested Working Concentration:
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0.5 µg/mL for Western blotting
0.2-0.5 µg/mL for Immunocytochemistry
1 µg/mL for In-Cell ELISA (0.1 µg/well)
1 µg/mL for Flow Cytometry
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Storage Conditions:
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Store at 4°C. Do not freeze.
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Country of Origin:
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USA
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WB Images
(click to enlarge)
Figure 1. Isolated mitochondria from human heart detected with (MSP05) anti-subunit E2 antibody.
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ICC Images
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Figure 2. Mitochondrial localization of PDH in cultured, normal human embryonic lung fibroblasts (strain MRC5) labeled with anti-PDH-E2 mAb 15D3G9C11 (MSP05) as the primary antibody (1 ug/ml) and an AlexaFluor® 488 goat anti-mouse IgG1 secondary antibody (2 ug/ml).
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IHC Images
(click to enlarge)
Figure 3. PDH subunit E2 immunohistochemistry in human cerebellum visualized with mAb MSP05. PDH subunit E2 immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells. Image kindly provided by Nichola Lax and Doug Turnbull, Mitochondrial Research Group, Newcastle University.
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Flow Images
(click to enlarge)
Figure 4. HL-60 cells were stained with 1 µg/mL PDH subunit E2 antibody (MSP05) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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CART
