Pyruvate dehydrogenase (PDH) subunit E2 monoclonal antibody
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Catalog No. MSP05
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$325.00 - 100 µg
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This antibody can also be purchased as part of a Sample Pack.
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UniProt Number:
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P10515
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Alternate Names:
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Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, M2 antigen complex 70 kDa subunit, Pyruvate dehydrogenase complex E2 subunit (Short name=PDC-E2), (Short name=PDCE2), (Short name=E2) Dihydrolipoamide S-acetyltransferase component of pyruvate dehydrogenase complex 70 kDa mitochondrial autoantigen of primary biliary cirrhosis (Short name=PBC)
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Structure and Function:
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The pyruvate dehydrogenase complex contains three different subunits including the dihydrolipoamide acetyltransferase (E2) subunit.
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Disease Associations:
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The E2 subunit of the PHD complex is associated with primary biliary cirrhosis in that patients diagnosed with this disease have autoantibodies for this subunit. This condition causes damage to liver cells and can result in cirrhosis of the liver. Lactic acidemia (PDH deficiency) is another condition associated with the E2 subunit. The clinical characterization of this disorder includes primary lactic acidosis as well as neurological problems, mainly episodic dystonia.
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Product Specifications
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Applications:
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Western blotting, Immunohistochemistry, Immunocytochemistry (heat-induced antigen-retrieval improves signal), and In-Cell ELISA
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Species Reactivity:
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human, bovine
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Host Species:
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mouse
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Isotype:
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IgG1, k
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Clone ID:
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15D3G9C11
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Concentration:
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1.0 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
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Suggested Working Concentration:
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0.5 µg/mL for Western blotting
0.2-0.5 µg/mL for Immunocytochemistry
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Storage Conditions:
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4°C
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Country of Origin:
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USA
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WB Images
(click to enlarge)
Figure 1. Isolated mitochondria from human heart detected with (MSP05) anti-subunit E2 antibody.
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ICC Images
(click to enlarge)
Figure 2. Mitochondrial localization of PDH in cultured, normal human embryonic lung fibroblasts (strain MRC5) labeled with anti-PDH-E2 mAb 15D3G9C11 (MSP05) as the primary antibody (1 ug/ml) and an AlexaFluor® 488 goat anti-mouse IgG1 secondary antibody (2 ug/ml).
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IHC Images
(click to enlarge)
Figure 3. PDH subunit E2 immunohistochemistry in human cerebellum visualized with mAb MSP05. PDH subunit E2 immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.
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