Pyruvate dehydrogenase (PDH) subunit E1 alpha monoclonal antibody

Catalog No. MSP07

$325.00 - 100 µg

UniProt Number: P08559
Alternate Names: Pyruvate dehydrogenase E1 component subunit alpha (somatic form), PDHE1-A type I, PDHA1, PDHE1A
Structure and Function: The pyruvate dehydrogenase complex contains three different subunits including the pyruvate dehydrogenase E1 subunit.The PDH complex is responsible for catalyzing the formation of acetyl-CoA and CO2 from pyruvate.
Disease Associations: Enzyme deficiency of the PDH E1 subunit of the PDH complex commonly results in lactic acidosis. There are a wide range of symptoms that characterize this disorder including neonatal death, developmental delay, seizures, ataxia, apnea and X-link Leigh Syndrome (LS). This syndrome presents itself with necrotic lesions that are located symmetrically in both sides the subcortical brain.


Product Specifications
Applications: Western blotting, Immunocytochemistry (heat-induced antigen-retrieval improves signal), Flow Cytometry
Species Reactivity: human, bovine, mouse, rat, Drosophila, C. elegans
Host Species: mouse
Isotype: IgG1, κ
Clone ID: 8D10E6
Concentration: 1.0 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
Suggested Working Concentration: 0.5-1.0 µg/mL for Western blotting
1 µg/mL for Immunocytochemistry
1 µg/mL for Flow Cytometry
Storage Conditions: Store at 4°C. Do not freeze.
Country of Origin: USA



WB Images

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Figure 1. Isolated mitochondria from human heart (lane 1), bovine heart (lane 2), rat heart (lane 3), mouse heart (lane 4), and HepG2 (lane 5) detected with (MSP07) anti-subunit E1 alpha antibody.

ICC Images

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Figure 2. Immunocytochemistry image of MSP07 stained human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the antibody (8D10E6, 1 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.

Flow Images

(click to enlarge)

Figure 3. HL-60 cells were stained with 1 µg/mL PDH subunit E1 alpha antibody (MSP07) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.



Downloadable Documents

   Technical Data Sheet

   Western blotting Protocol

   Immunocytochemistry Protocol

   Flow Cytometry Protocol

   PDH Playbook

   MSDS Sodium Azide



Published Studies Using This Product: Brys et al., 2010. Disruption of insulin signalling preserves bioenergetic competence of mitochondria in ageing Caenorhabditis elegans.

Glushakova et al., 2009. AAV3-mediated transfer and expression of the pyruvate dehydrogenase E1 alpha subunit gene causes metabolic remodeling and apoptosis of human liver cancer cells.

Cox et al., 2009. Clueless, a conserved Drosophila gene required for mitochondrial subcellular localization, interacts genetically with parkin.

Stenson et al., 2009. Activation of liver X receptor regulates substrate oxidation in white adipocytes.

Ostergaard et al., 2009. Four novel PDHA1 mutations in pyruvate dehydrogenase deficiency.

Greiss et al., 2008. C. elegans SIR-2.1 translocation is linked to a proapoptotic pathway parallel to cep-1/p53 during DNA damage-induced apoptosis.


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