Pyruvate dehydrogenase (PDH) subunit E1 alpha monoclonal antibody
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Catalog No. MSP07
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$325.00 - 100 µg
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This antibody can also be purchased as part of a Sample Pack.
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UniProt Number:
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P08559
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Alternate Names:
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Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, PDHE1-A type I
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Structure and Function:
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The pyruvate dehydrogenase complex contains three different subunits including the pyruvate dehydrogenase E1 subunit.The PDH complex is responsible for catalyzing the formation of acetyl-CoA and CO2 from pyruvate.
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Disease Associations:
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Enzyme deficiency of the PDH E1 subunit of the PDH complex commonly results in lactic acidosis. There are a wide range of symptoms that characterize this disorder including neonatal death, developmental delay, seizures, ataxia, apnea and X-link Leigh Syndrome (LS). This syndrome presents itself with necrotic lesions that are located symmetrically in both sides the subcortical brain.
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Product Specifications
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Applications:
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Western blotting and Immunocytochemistry (heat-induced antigen-retrieval improves signal)
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Species Reactivity:
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human, bovine, mouse, rat, Drosophila melanogaster and C. elegans
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Host Species:
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mouse
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Isotype:
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IgG1, k
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Clone ID:
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8D10E6
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Concentration:
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1.0 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
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Suggested Working Concentration:
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0.5-1.0 µg/mL for Western blotting
1 µg/mL for Immunocytochemistry
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Storage Conditions:
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4°C
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Country of Origin:
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USA
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WB Images
(click to enlarge)
Figure 1. Isolated mitochondria from human heart (lane 1), bovine heart (lane 2), rat heart (lane 3), mouse heart (lane 4), and HepG2 (lane 5) detected with (MSP07) anti-subunit E1 alpha antibody.
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ICC Images
(click to enlarge)
Figure 2. Mitochondrial localization of PDH in cultured, normal human fibroblasts labeled with anti-PDH-E1 alpha mAb 8D10E6 (MSP07) as the primary antibody and an Alexa Fluor® 488 goat anti-mouse secondary antibody.
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