Pyruvate dehydrogenase (PDH) subunit E1 alpha monoclonal antibody
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Catalog No. MSP07
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$325.00 - 100 µg
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UniProt Number:
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P08559
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Alternate Names:
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Pyruvate dehydrogenase E1 component subunit alpha (somatic form), PDHE1-A type I, PDHA1, PDHE1A
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Structure and Function:
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The pyruvate dehydrogenase complex contains three different subunits including the pyruvate dehydrogenase E1 subunit.The PDH complex is responsible for catalyzing the formation of acetyl-CoA and CO2 from pyruvate.
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Disease Associations:
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Enzyme deficiency of the PDH E1 subunit of the PDH complex commonly results in lactic acidosis. There are a wide range of symptoms that characterize this disorder including neonatal death, developmental delay, seizures, ataxia, apnea and X-link Leigh Syndrome (LS). This syndrome presents itself with necrotic lesions that are located symmetrically in both sides the subcortical brain.
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Product Specifications
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Applications:
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Western blotting, Immunocytochemistry (heat-induced antigen-retrieval improves signal), Flow Cytometry
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Species Reactivity:
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human, bovine, mouse, rat, Drosophila, C. elegans
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Host Species:
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mouse
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Isotype:
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IgG1, κ
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Clone ID:
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8D10E6
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Concentration:
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1.0 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
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Suggested Working Concentration:
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0.5-1.0 µg/mL for Western blotting
1 µg/mL for Immunocytochemistry
1 µg/mL for Flow Cytometry
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Storage Conditions:
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Store at 4°C. Do not freeze.
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Country of Origin:
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USA
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WB Images
(click to enlarge)
Figure 1. Isolated mitochondria from human heart (lane 1), bovine heart (lane 2), rat heart (lane 3), mouse heart (lane 4), and HepG2 (lane 5) detected with (MSP07) anti-subunit E1 alpha antibody.
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ICC Images
(click to enlarge)
Figure 2. Immunocytochemistry image of MSP07 stained human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the antibody (8D10E6, 1 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
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Flow Images
(click to enlarge)
Figure 3. HL-60 cells were stained with 1 µg/mL PDH subunit E1 alpha antibody (MSP07) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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CART
