MitoSciences: MSP31 Pyruvate dehydrogenase (PDH) Protein Quantity Dipstick Assay Kit

Enzymes > Pyruvate dehydrogenase > PDH Protein Quantity Dipstick Assay Kit

Pyruvate dehydrogenase (PDH) Protein Quantity Dipstick Assay Kit

Catalog No. MSP31

$325.00 - 30 tests
$595.00 - 90 tests

Product Description

This kit contains dipsticks and necessary components to quantify the activity of the PDH enzyme complex (pyruvate dehydrogenase) from human, bovine, mouse, rat, and pig samples.

Learn more about Dipstick Protein Quantity Assays >

Specifications

Data

Documentation

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Product Specifications
Species Reactivity:	human, rat, mouse, bovine
Storage Conditions:	Store dipsticks and gold-conjugated secondary antibody at room temperature out of direct sunlight in their provided containers. Store buffers at 4°C.
Country of Origin:	USA

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Specifications

Data

Documentation

Citations

Figure 1

(click to enlarge)
Figure 1. An example using MSP31 to quantify PDH levels using various concentration of rat tissue protein extract.

Shown is a 1:2 dilution series using a positive control sample. Approximately 7 to 8 dipsticks are suitable for covering the entire working range and the blank for background levels. In this example the dilution series starts with 20 µg of rat heart tissue extract. A one-site hyperbola line was generated for best-fit analysis using GraphPad.

Figure 2

(click to enlarge)
Figure 2. An example using MSP31 to quantify PDH levels using various concentration of rat tissue protein extract.

Based on the above standard curve values, 18 µg (control - undiluted), 9 µg (sample A - diluted 1:2) and 1.8 µg (sample B - diluted 1:10) of extract were loaded to each dipstick. Two dipsticks per sample were run (typical intra-assay precision is 90-98%). Using GraphPad software, the signal intensity from the standard curve was interpolated and the quantity of PDH in samples A and B was determined. Based on the above analysis, the unknown samples, A and B, had 48% and 9% of control PDH levels, respectively.

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