MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail

Catalog No. MS604

$575.00 - 300 µg
$945.00 - 600 µg

Product Description

Contains 5 mAbs, one each against CI subunit NDUFB8 (MS105), CII-30kDa (MS203), CIII-Core protein 2 (MS304) CIV subunit I (MS404) and CV alpha subunit (MS507) as an optimized premixed cocktail. The kit is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from mouse, rat, human, or bovine sources.

Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.

The mAbs in the cocktail were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used (see protocols). Note: Mouse tissue samples can easily be contaminated with antibodies from the animal's blood. To avoid such background bands, use MS604 in conjunction with a secondary against native antibodies e.g. True blot ( Also, COXI is a highly hydrophobic protein and appears as a broad band at ~35 kDa (not at its true molecular weight at 57 kDa). It is very sensitive to heating. Therefore, the samples, including the positive control, should not be heated over 50°C before loaded on the gel.


Product Specifications
Targets: • Complex I subunit NDUFB8 - "CI-20" ~ 20kD
• Complex II subunit 30kDa - "CII-30" ~ 30kD
• Complex III subunit Core 2 - "CIII-core2" ~ 47kD
• Complex IV subunit I - "CIV-I" ~ 39kD
• ATP synthase subunit alpha - "CV-alpha" ~ 53kD
Species Reactivity: mouse, rat, human, bovine
Storage Conditions: 4°C
Country of Origin: USA


Figure 1

(click to enlarge)

Figure 1. Rat liver mitochondria labeled with MS604. The sample in lane 1 was kept at room temperature, whereas the remaining three samples were heated to 37°C, 50°C, and 100°C, respectively.



Downloadable Documents

   Technical Data Sheet


   MSDS Sodium Azide



Published Studies Using This Product: Vetterli et al., 2010. Resveratrol potentiates glucose-stimulated insulin secretion in INS-1E beta-cells and human islets through Sirt1 dependent mechanism.

Hoy et al., 2010. Adipose Triglyceride Lipase-Null Mice Are Resistant to High-Fat Diet-Induced Insulin Resistance Despite Reduced Energy Expenditure and Ectopic Lipid Accumulation.

Demaria et al., 2010. A STAT3-mediated metabolic switch is involved in tumour transformation and STAT3 addiction.

Rikka et al., 2010. Bnip3 impairs mitochondrial bioenergetics and stimulates mitochondrial turnover.

Dorado et al., 2010. Onset and organ-specificity of Tk2 deficiency depends on Tk1 down-regulation and transcriptional compensation.

Hlavackova et al., 2010. Up-regulation and redistribution of protein kinase C-δ in chronically hypoxic heart.

Lee-Young et al., 2010. Endothelial Nitric Oxide Synthase is Central to Skeletal Muscle Metabolic Regulation and Enzymatic Signaling during Exercise In Vivo.

Faerber et al., 2010. Induction of heart failure by minimally invasive aortic constriction in mice: Reduced peroxisome proliferator-activated receptor gamma coactivator levels and mitochondrial dysfunction.

Yang et al., 2010. NAD+-dependent deacetylase SIRT3 regulates mitochondrial protein synthesis by deacetylation of the ribosomal protein MRPL10.

Rached et al., 2010. FoxO1 expression in osteoblasts regulates glucose homeostasis through regulation of osteocalcin in mice.

Yang et al., 2010. Analysis of mouse models of cytochrome c oxidase deficiency owing to mutations in Sco2.

Murray et al., 2009. Deterioration of physical performance and cognitive function in rats with short-term high-fat feeding.

Malmgren et al., 2009. Tight coupling between glucose and mitochondrial metabolism in clonal beta-cells is required for robust insulin secretion.

Li et al., 2009. Transient oxidative stress damages mitochondrial machinery inducing persistent beta-cell dysfunction.

Gispert et al., 2009. Parkinson phenotype in aged PINK1-deficient mice is accompanied by progressive mitochondrial dysfunction in absence of neurodegeneration.

Metukuri et al., 2009. Expression and subcellular localization of BNIP3 in hypoxic hepatocytes and liver stress.

Naviaux et al., 2009. Retained features of embryonic metabolism in the adult MRL mouse.

López et al., 2009. Unbalanced deoxynucleotide pools cause mitochondrial DNA instability in thymidine phosphorylase-deficient mice.

Akman et al., 2008. Thymidine kinase 2 (H126N) knockin mice show the essential role of balanced deoxynucleotide pools for mitochondrial DNA maintenance.

Bambrick et al., 2008. Mitochondrial dysfunction in mouse trisomy 16 brain.

Sun et al., 2007. Mitochondrial dysfunction precedes neurodegeneration in mahogunin (Mgrn1) mutant mice.

Boengler et al., 2007. Mitochondrial respiration and membrane potential after low-flow ischemia are not affected by ischemic preconditioning.

Yin et al., 2006. Proteomic analysis reveals higher demand for antioxidant protection in embryonic stem cell-derived smooth muscle cells.


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Companion Products:
• Complex I subunit NDUFB8
  monoclonal antibody

• Complex II subunit 30 kDa Ip
  monoclonal antibody

• Complex III subunit Core 2
  monoclonal antibody

• Complex IV subunit 1
  monoclonal antibody

• ATP synthase subunit alpha
  monoclonal antibody

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