Products for Evaluating Oxidative Phosphorylation
Overview
Oxidative phosphorylation (OXPHOS) is the final metabolic pathway of cellular respiration. It takes place in the inner membrane of the mitochondria where NADH and FADH2 are used in the electron transfer chain to pump H+ across the membrane against a proton gradient. ATP synthase molecules embedded in the membrane allow protons to pass through, generating ATP from ADP as protons pass down the gradient.
Altered levels of assembly in the OXPHOS complexes can arise from mutations in individual subunits, mutations in assembly factors for the complexes, mtDNA depletion, or as a result of physiological and or pathological changes (e.g. hormone treatment, exercise, diet or oxidative stress).
We offer kits that allow researchers to evaluate the functioning of the OXPHOS pathway by screening for deficiencies in all five of the OXPHOS complexes using either Western blotting or immunocytochemistry.
Research Kits
MS601 Human Total OXPHOS Complexes Detection Kit
Contains a total of 360 µg or 720 µg of antibodies and is a premixed solution of 5 monoclonal antibodies against a subunit of each of the 5 OXPHOS complexes in mitochondrial preparations from human sources. Human heart mitochondria are also included as a Western blotting control.
MS602 OXPHOS/PDH Immunocytochemistry Kit
A cocktail of 220 µg antibodies comprised of six mAbs packaged individually. Each antibody is against a labile subunit of each of the 5 OXPHOS complexes and PDH. Each mAb is provided in a 50 µl aliquot at a 200x concentration, to allow reconstitution of each mAb in 10 ml working volume.
MS602a OXPHOS/PDH Immunocytochemistry Kit
Includes the same components as MS602 (above), but additionally contains 60 µg mAb against porin, and 120 µg against Complex V α subunit, and the required secondary antibodies for simultaneous 2 dye staining of samples.
MS604 Rodent Total OXPHOS Complexes Detection Kit
Contains a total of 300 µg or 600 µg of antibodies and is a premixed solution of 5 monoclonal antibodies against a subunit of each of the 5 OXPHOS complexes in mitochondrial preparations from rodent sources.
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