MSA31 MitoProfile Dipstick Assay Kit
for Apoptosis-Inducing Factor (AIF) Quantity


30 Dipstick Kit: $325.00
60 Dipstick Kit: $545.00
Please contact us for volume discounts.


Brief Description & Uses

Contains 30 or 60 dipsticks and necessary components to quantify the amount of Apoptosis-Inducing Factor (AIF) from human sample material. The kit includes sufficient materials to generate a standard curve and prepare samples for analysis.

AIF is a flavoprotein (67 kDa) localized in the inter-membrane space. It is a multi-functional protein with a vital oxidoreductase function (required for cell survival), an anti-oxidant activity, an assembly function in Complex I (NADH-ubiquinone oxidoreductase complex) formation and an apoptogenic function. AIF has a major role in the caspase-independent apoptosis process. It translocates to the nucleus after mitochondrial release, leading to chromatin condensation and DNA fragmentation.

The process of apoptosis, the main type of programmed cell death, is an important function in cell termination, cell homeostasis, tissue development and lymphocyte interactions.

For a detailed overview of the MitoProfile Dipstick Technology please click here.


Immunochromatographic Reader

While visual measurements and scanning on a flatbed scanner can both be used to evaluate our dipsticks, the most effective quantitative results are derived when using an immunochromatographic reader that has been specifically designed to read conjugated gold lateral flow devices.

The reader that we recommend is the Hamamatsu ICA-1000, which is a simple PC-based system for measuring and analyzing our dipstick products. We have an agreement in place with Hamamatsu that allows us to offer this reader directly to our customers. To learn more about this reader, or to order one, please click here to visit the product page.


Specifications

Product: MitoProfile Dipstick Assay Kit for Apoptosis-Inducing Factor (AIF) Quantity
Catalog #: MSA31
Kit Components: Dipsticks with mAb, gold-conjugated antibody and buffers.
mAb Clone ID: 7F7AB10 (dipsticks)
6C4CG (dried gold-conjugated in micro-titer plate)
Applications: Quantitative analysis of AIF levels
Antigen Species Cross-Reactivity: Human
Storage: Store dipsticks and plate at room temperature out of direct sunlight in their provided containers. Store buffer A and buffer C at 4C. Store buffer B at -20C.
Country of Origin: USA



Product References

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References for AIF




Images

The above results show the accuracy of the AIF dipstick kit (MSA31) when measuring reduced amounts of AIF when a sample is diluted in 50% increments. HepG2 cell extract was diluted 1:2 and 1:4 and equal volumes of extract were loaded per dipstick. Graphical analysis was performed using values interpolated from a standard curve. All analysis was done with the MS1000 dipstick reader and GraphPad software.




Downloadable Information
MSA31 Specifications


Additional Information
Product Images
Product References
Protein References


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Detergents

MSA09 AIF monoclonal antibody
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