MS821 Mouse Heart mitochondrial sample for
Immunocapture control and Blue Native PAGE
$300.00 (2 mg)
Please contact us for volume discounts.
Brief Description & Uses
2 mg of mouse heart mitochondria in iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE.
Specifications
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Product:
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Mouse Heart Mitochondrial membranes for
Immunocapture control and Blue Native PAGE
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Catalog #:
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MS821
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Lot #:
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Concentration:
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2 mg of purified mouse heart mitochondrial membranes resuspended in 400 µl of heart mitochondria resuspension buffer (10 mM Tris.HCl, pH 7.8, 0.2 M sucrose, 0.2 mM EDTA, 1 mM PMSF).
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Suggested Working Concentration:
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1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant. For more detail see Mitosciences suggested protocols.
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Applications:
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Immunocapture, however could also be used for electrophoresis and Western blotting.
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Storage:
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Shipped at 4°C. Store at -20°C after receipt
or at -80°C for long term.
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Country of Origin:
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USA
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