MS601 MitoProfile Human Total OXPHOS
Complexes Detection Kit


360 g Kit: $595.00
720 g Kit: $945.00
Please contact us for volume discounts.


Brief Description & Uses

Contains 360 g or 720 g of 5 mAbs against CI subunit NDUFB8 (MS105), CII-30kDa (MS203), CIII-Core protein 2 (MS304) CIV subunit II (MS405) and CV α subunit (MS507) respectively as an optimized premixed cocktail. (MS105 was previously listed as likely CI-ND6). The kit is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from human sources. Human heart mitochondria are also included as a Western Blotting control.

Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.

The mAbs in the cocktail were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used (see protocols). Examples of the use of kit MS601 in studies of patients with mitochondrial diseases can be seen by clicking here.


Specifications

Product: MitoProfile Human Total OXPHOS
Complexes Detection Kit
Kit Components: Premixed cocktail containing 5 monoclonal antibodies (see Table below), HHM control.
Catalog #: MS601
Lot #:
Host Species: Mouse
Concentration: 1.5 mg/mL in HEPES-Buffered Saline (HBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: The antibody cocktail (1.5mg/mL) should be diluted 200x to a final working concentration of 7.2 g/mL for Western blotting
Applications: Western blotting detection of enzyme purity or for OXPHOS complex deficiency detection.
Antigen Species Cross-Reactivity: Human
Storage: 4C
Country of Origin: USA



Product Components

ANTIGEN CAT # CLONE ID AMOUNT
(g)
FINAL CONC.
(g/ml)
IMMUNOGEN
C-I-NDUFB8 MS105 20E9DH10C12 50 1 Bovine Complex I
C-II-30 (FeS) MS203 21A11AE7 250 5 Bovine Heart Complex II
C-III-Core 2 MS304 13G12AF12BB11 5 0.1 Bovine Heart Complex II,III
C-IV-COXII MS405 12C4F12 50 1 Human Complex IV, subunit II
C-V-α MS507 15H4C4 5 0.1 Bovine Complex V



Product References




References for Oxidative Phosphorylation

There are no references currently posted for this product.




Images

MS601 Western blotting results


Downloadable Information
MS101 Specifications Western Blotting Protocol


Additional Information
Product Components
Product Images
Product References
Protein References


Related Products
Western Blotting Controls

Secondary Antibodies
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