MS503 ATP synthase (Complex V) subunit β
monoclonal antibody


$295.00 (100 g)

Please contact us for volume discounts.
This product can also be ordered as part of a sample pack.



Brief Description & Uses

100 g of monoclonal antibody reactive against the 52 kDa F1F0 ATP synthase (Complex V) β subunit. MS503 also labels β in immunocytochemical applications. Isolated mitochondria are available from several species for use as control samples. To see the complete list of control samples available from MitoSciences, click here.

An important recent finding is that F1F0 is an ectopic protein in many cells and tissues in addition to its location within mitochondria. In endothelial cells F1F0 acts as an angiostatin receptor. Both synthesis and hydrolysis activity of the ectopic F1F0 are inhibited by angiostatin. The β mAb above (MS503) has been shown to act as an angiostatin mimetic and to inhibit the proliferation of endothelial cells. The anti β subunit mAb (MS503) is able to detect plasma membrane F1F0. More recently it has been reported that in liver cells this protein complex is the HDL receptor. Importantly not all cells have F1F0 on their surface; there is no reactivity of this antibody with the surface of MRC-5 fibroblast cells.


Specifications

Product: ATP synthase (Complex V) subunit β
monoclonal antibody
Catalog #: MS503
Lot #:
Clone ID: 3D5AB1
Host Species and Isotype: Mouse IgG1, κ
Immunogen: Human Heart Mitochondria
Concentration: 1 mg/ml in Dulbecco’s Phosphate-Buffered Saline (PBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: 0.5 g/ml for Western blotting, 1-2 g/ml for Immunocytochemistry
Applications: Western blotting, Immunocytochemistry
Antigen Species Cross-Reactivity: human, bovine, mouse, rat and C. elegans
Storage: 4C
Country of Origin: USA



Product References




References for ATP synthase (Complex V) subunit β




Images




Complex V subunit ? Immunocytochemistry Image


Downloadable Information
MS503 Specifications Western Blotting Protocol Immunocytochemistry Protocol


Additional Information
Product Images
Product References
Protein References


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