MS501c ATP synthase (Complex V)
Immunocapture monoclonal antibody


$295.00 (100 g)

Please contact us for volume discounts.


Brief Description & Uses

100 g monoclonal antibody which can immunocapture up to 50 g of ATP synthase (Complex V) from heart mitochondria. Isolated mitochondria are available from several species for use as control samples. To see the complete list of control samples available from MitoSciences, click here.

The ATP synthase (Complex V) immunocapture kit allows isolation of the ATP synthase complex (E.C. 3.6.3.14) from small amounts of tissue. This facilitates subsequent analysis of assembly state and activity. Thus the enzyme retains olygomycin sensitive ATP hydrolysis activities after isolation. Finally, the extent of post translational modifications including oxidative damage can be readily analyzed by proteomic approaches or antibody detection of these modifications. Potential uses for the ATP synthase (Complex V) immunocapture kit include but are not limited to examining alterations of Complex V subunits in inherited mitochondrial diseases, Alzheimer's disease, angiogenesis, hypertension and aging.


Specifications

Product: ATP synthase (Complex V) Immunocapture
monoclonal antibody
Catalog #: MS501c
Lot #:
Clone ID: 12F4AD8AF8
Host Species and Isotype: Mouse IgG2b, κ
Immunogen: Bovine Complex V
Concentration: 1 mg/ml in Dulbecco’s Phosphate-Buffered Saline (PBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: 100 g mAb can capture up to 50 g ATP synthase (Complex V) from 1 mg solubilized bovine heart mitochondria.
Applications: Immunoprecipitation
Antigen Species Cross-Reactivity: human, bovine, rat and mouse
Storage: 4C
Country of Origin: USA



Product References

Toxicology In Vitro. 2007 Aug;21(5):902-11.
"Target identification of drug induced mitochondrial toxicity using immunocapture based OXPHOS activity assays."


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References for ATP synthase (Complex V)




Images



Downloadable Information
MS501c Specifications Immunocapture Protocol


Additional Information
Product Images
Product References
Protein References


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Detergents

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MS501 ATP synthase (Complex V) Immunocapture Kit
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