MS501 MitoProfile ATP synthase (Complex V)
Immunocapture Kit


250 g Kit: $375.00
500 g Kit: $675.00
750 g Kit: $875.00
Please contact us for volume discounts.


Brief Description & Uses

Contains a total of 250 g, 500 g or 750 g of monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture up to ~125 g, ~250 g or ~500 g respectively of ATP synthase (Complex V) from heart mitochondria. Isolated mitochondria are available from several species for use as control samples. To see the complete list of control samples available from MitoSciences, click here.

The MitoProfile ATP synthase (Complex V) Immunocapture Kit allows isolation of the ATP synthase complex (E.C. 3.6.3.14) from small amounts of tissue. This facilitates subsequent analysis of assembly state and activity. Thus the enzyme retains olygomycin sensitive ATP hydrolysis activities after isolation. Finally, the extent of post translational modifications including oxidative damage can be readily analyzed by proteomic approaches or antibody detection of these modifications. Potential uses for the ATP synthase (Complex V) immunocapture kit include but are not limited to examining alterations of Complex V subunits in inherited mitochondrial diseases, Alzheimer’s disease, angiogenesis, hypertension and aging.


Specifications

Product: MitoProfile ATP synthase (Complex V) Immunocapture Kit

Kit Components:

250 g of antibody crosslinked to protein G-agarose beads, BHM control.

Catalog #: MS501
Lot #:
Clone ID: 12F4AD8AF8
Host Species and Isotype: Mouse IgG2b, κ
Immunogen: Bovine Complex V
Concentration: 250 g of capture antibody crosslinked to 62.5 l matrix in 400 l HEPES-Buffered Saline (HBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: 10 l matrix capture approximately 20 g ATP synthase (Complex V) from 1 mg solubilized bovine heart mitochondria.
Applications: Immunoprecipitation
Antigen Species Cross-Reactivity: human, bovine, rat and mouse
Storage: 4C
Country of Origin: USA



Product References




References for ATP synthase (Complex V)




Images



Downloadable Information
MS501 Specifications Immunocapture Protocol


Additional Information
Product Images
Product References
Protein References


Related Products
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Detergents

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MS501c ATP synthase (Complex V) Immunocapture mAb
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