MS403 Complex IV subunit SURF1
monoclonal antibody


$295.00 (100 g)

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This product can also be ordered as part of a sample pack.



Brief Description & Uses

100 g of monoclonal antibody reactive against the 33 kDa complex IV assembly factor SURF1. Cellular levels of SURF1 are highest in growing cultured cells.

SURF1 is an important assembly factor for cytochrome c oxidase which is thought to participate in the folding of subunit II and parallel Cu2+ insertion into this subunit. Mutations of SURF1, particularly ones that result in an absence or near absence of the polypeptide, are the leading cause of cytochrome c oxidase deficiency.

The anti-SURF1 mAb reacts specifically in human and bovine tissues. The protein is easily detected in cells rapidly dividing their mitochondria and has the highest levels in cells impaired in COX assembly. The levels of SURF1 are always low and in some cases neglible, even in rapidly dividing fibroblast cell cultures. Levels are lowest in mature tissues such as heart or liver; the levels of SURF1 are in fact so low that a large amount of tissue material must be used that the specificity is not as good as in cells. The low copy number is consistent with it acting to catalyze Complex IV assembly. In cells where cytochrome c oxidase assembly is incomplete because of mutations in genes for structural subunits of the enzyme SURF1 is significantly over-expressed. This is evident in Rho0 fibroblasts which are missing mtDNA and hence cannot synthesize subunits I, II and III of cytochrome c oxidase.


Specifications

Product: Complex IV subunit SURF1
monoclonal antibody
Catalog #: MS403
Lot #:
Clone ID: 21H2BG4
Host Species and Isotype: Mouse IgG1, κ
Immunogen: Recombinant human SURF1
Concentration: 1 mg/ml in Dulbecco’s Phosphate-Buffered Saline (PBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: 1.0 g/ml for Western blotting
Applications: Western blotting
Antigen Species Cross-Reactivity: human
Storage: 4C
Country of Origin: USA



Product References




References for Complex IV subunit SURF1

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Images

Complex IV subunit SURF1 Western Blotting Image


Downloadable Information
MS101 Specifications Western Blotting Protocol Research Playbook


Additional Information
Product Images
Product References
Protein References


Related Products
Western Blotting Controls

Secondary Antibodies
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