MS401 MitoProfile Complex IV Immunocapture Kit


250 g Kit: $375.00
500 g Kit: $675.00
750 g Kit: $875.00
Please contact us for volume discounts.


Brief Description & Uses

250 g, 500 g or 750 g monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 g, ~50 g or ~75 g respectively of Complex IV from heart mitochondria. Isolated mitochondria are available from several species for use as control samples. To see the complete list of control samples available from MitoSciences, click here.

The MitoProfile Complex IV Immunocapture Kit allows isolation of the cytochrome c oxidase complex (E.C. 1.9.3.1) from small amounts of tissue. This facilitates subsequent analysis of both assembly state and accrued post-translational modifications of all 13 subunits of the enzyme. The immunoprecipitated Complex IV shows cytochrome c oxidase activity while bound to the beads and this activity is fully sensitive to cyanide. Uses for the Complex IV immunocapture kit include but are not limited to examining alterations of Complex IV subunits in inherited mitochondrial diseases, Alzheimer’s disease, schizophrenia and cancer.


Specifications

Product: MitoProfile Complex IV Immunocapture Kit

Kit Components:

250 g of antibody crosslinked to protein G-agarose beads, BHM control.

Catalog #: MS401
Lot #:
Clone ID: 31E91B82G9
Host Species and Isotype: Mouse IgG1, κ
Immunogen: Bovine Complex IV
Concentration: 250 g of capture antibody crosslinked to 25 l matrix in 400 l Dulbecco’s Phosphate-Buffered Saline (PBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: 10 l matrix capture approximately 10 g complex IV (COX) from 1 mg solubilized bovine heart mitochondria.
Applications: Immunoprecipitation
Antigen Species Cross-Reactivity: human and bovine
Storage: 4C
Country of Origin: USA



Product References




References for Complex IV (cytochrome c oxidase)

There are no references currently posted for this product.




Images

Complex IV immunoprecipitation results


Downloadable Information
MS401 Specifications Immunocapture Protocol Research Playbook


Additional Information
Product Images
Product References
Protein References


Related Products
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Secondary Antibodies

Detergents

MS401c Complex IV Immunocapture mAb

MS402 Complex IV Immunocapture Kit for Rodent
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