MS201 MitoProfile Complex II Immunocapture Kit


250 g Kit: $375.00
500 g Kit: $675.00
750 g Kit: $875.00
Please contact us for volume discounts.


Brief Description & Uses

250 g, 500 g or 750 g monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 g, ~26 g or ~39 g respectively of Complex II from heart mitochondria. Isolated mitochondria are available from several species for use as control samples. To see the complete list of control samples available from MitoSciences, click here.

The MitoProfile Complex II Immunocapture Kit allows isolation of the succinate dehydrogenase complex (E.C. 1.3.5.1) from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for the Complex II immunocapture kit include research on aging genetic mitochondrial disease, and various cancers due to mutations in this enzyme complex such as paragangliomas and phaeo-chromocytomas.


Specifications

Product: MitoProfile Complex II Immunocapture Kit

Kit Components:

250 g of antibody crosslinked to protein G-agarose beads, BHM control.

Catalog #: MS201
Lot #:
Clone ID: 4H12BG12AG2
Host Species and Isotype: Mouse IgG1, κ
Immunogen: Bovine Heart Complex II, with traces of Complex III
Concentration: 250 g of capture antibody crosslinked to 25 l matrix in 400 l HEPES-Buffered Saline (HBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: 10 l matrix capture approximately 5 g complex II from 1 mg solubilized bovine heart mitochondria.
Applications: Immunoprecipitation
Antigen Species Cross-Reactivity: human, bovine, mouse and rat
Storage: 4C
Country of Origin: USA



Product References




References for Complex II (Succinate ubiquinone oxidoreductase)

There are no references currently posted for this product.




Images



Downloadable Information
MS201 Specifications Immunocapture Protocol


Additional Information
Product Images
Product References
Protein References


Related Products
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Secondary Antibodies

Detergents

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MS201c Complex II Immunocapture mAb
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