MS133 MitoProfile Dipstick Assay Kit for Rodent Complex I Quantity


30 Dipstick Kit: $325.00
60 Dipstick Kit: $545.00
Please contact us for volume discounts.


Brief Description & Uses

Contains 30 or 60 dipsticks and necessary components to quantify the levels of the fully assembled Complex I enzyme complex (NADH ubiquinone oxidoreductase) from mouse and rat samples. The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.

For a detailed overview of MitoSciences Dipstick Assay Technology please click here.


Immunochromatographic Reader

While visual measurements and scanning on a flatbed scanner can both be used to evaluate our dipsticks, the most effective quantitative results are derived when using an immunochromatographic reader that has been specifically designed to read conjugated gold lateral flow devices.

The reader that we recommend is the Hamamatsu ICA-1000, which is a simple PC-based system for measuring and analyzing our dipstick products. We have an agreement in place with Hamamatsu that allows us to offer this reader directly to our customers. To learn more about this reader, or to order one, please click here to visit the product page.


Specifications

Product: MitoProfile Dipstick Assay Kit for Rodent Complex I Quantity
Catalog #: MS133
Kit Components: Dipsticks, dried Complex I gold-conjugated antibody, and buffers.
mAb Clone ID: 18G12BC2 (dipsticks)
6E1BH7 (dried gold-conjugated Complex I subunit GRIM-19 monoclonal antibody)
Applications: Quantitative analysis of Complex I
Antigen Species Cross-Reactivity: Mouse, Rat
Storage: Store dipsticks and gold-conjugated secondary antibody at room temperature out of direct sunlight in their provided containers. Store buffers at 4C.
Country of Origin: USA



Product References




References for Complex I Dipstick Assays

There are no references currently posted for this product.



Images

The above results show the clear visual difference between a sample with normal levels of Complex I enzyme complex compared to patients with defects in Complex I assembly (patient 1 and 2) or cells depleted of mitochondrial proteins (rho0 cells). Also, we can see that the dipsticks are specific since they detect no alteration in Complex I assembly from a Complex IV patient. The dipstick results are both quantitative and highly reproducible (intra-assay error less than 10%). The developed dipsticks can be analyzed with a simple flat-be scanner and common imaging software or, for sensitive and reproducible results, a dipstick reader can be utilized (our suggested reader: Hamamatsu ICA-1000).



Downloadable Information
MS133 Specifications


Additional Information
Product Images
Product References
Protein References


Related Products
MS130 MitoProfile Dipstick Assay Kit for Complex I Activity

MS131 MitoProfile Dipstick Assay Kit for Human Complex I Quantity

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