MS101 MitoProfile Complex I Immunocapture Kit


250 g Kit: $375.00
500 g Kit: $675.00
750 g Kit: $875.00
Please contact us for volume discounts.


Brief Description & Uses

250 g, 500 g or 750 g monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~60 g, ~120 g or ~180 g respectively of Complex I (NADH dehydrogenase) from heart mitochondria. Isolated mitochondria are available from several species for use as control samples. To see the complete list of control samples available from MitoSciences, click here.

The MitoProfile Complex I Immunocapture Kit allows isolation of the NADH ubiquinone oxidoreductase complex (E.C. 1.6.5.3) from small amounts of tissue. This facilitates subsequent analysis of assembly state and activity. Thus the enzyme retains NADH-ferricyanide, NADH-CoQ1 and NADH hexaaminoruthenium reductase activities after isolation, and with added lipids it also shows significant rotenone sensitivity.

Finally, the extent of post translational modifications including oxidative damage can be readily analyzed by proteomic approaches or antibody detection of these modifications. Uses for the Complex I immunocapture kit include but are not limited to examining alterations of Complex I subunits in inherited mitochondrial diseases, Parkinson's disease, Alzheimer's disease, ALS, schizophrenia, and aging.


Specifications

Product: MitoProfile Complex I Immunocapture Kit
Kit Components: 250 g of antibody crosslinked to protein G-agarose beads, BHM control.
Catalog #: MS101
Lot #:
Clone ID: 18G12
Host Species and Isotype: Mouse IgG2b, κ
Immunogen: Bovine Heart Complex I
Concentration: 250 g of capture antibody crosslinked to 25 l matrix in 400 l HEPES-Buffered Saline (HBS) with 0.02% azide as a preservative.
Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Suggested Working Concentration: 10 l matrix capture approximately 25 g complex I from 1 mg solubilized bovine heart mitochondria.
Applications: Immunoprecipitation
Antigen Species Cross-Reactivity: human, bovine, rat and mouse
Storage: 4C
Country of Origin: USA



Product References




References for Complex I (NADH ubiquinone oxidoreductase)




Images



Downloadable Information
MS101 Specifications Immunocapture Protocol


Additional Information
Product Images
Product References
Protein References


Related Products
Immunocapture Controls

Secondary Antibodies

Detergents

MS851 Isolation Kit for Rodent Tissue

MS101c Complex I Immunocapture mAb
Home - About Us - Products - Support - Resources - Careers - Contact Us - Shopping Cart

Sales & Customer Support:
1-800-910-6486

[email protected]

Copyright 2004-2008 MitoSciences Inc. All rights reserved.