Mitofilin monoclonal antibody

Catalog No. MSM02

$325.00 - 100 µg


Product Specifications
Applications: Western blotting, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, In-Cell ELISA, Flow Cytometry
Species Reactivity: human
Host Species: mouse
Isotype: IgG1, κ
Clone ID: 2E4AD5
Concentration: 1.0 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
Suggested Working Concentration: 2 µg/mL for Western blotting
1-5 µg/mL for Immunocytochemistry
4 µg/mL for In-Cell ELISA (0.4 µg/well)
1 µg/mL for Flow Cytometry
Storage Conditions: Store at 4°C. Do not freeze.
Country of Origin: USA



ICC Images

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Figure 1. Mitochondrial localization of mitofilin visualized by immunocytochemistry using the anti-mitofilin mAb 2E4AD5 (MSM02). Cultured human fibroblasts were fixed, permeabilized and then labeled with MSM02 followed by a fluorescent goat-anti-mouse IgG.

IHC Images

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Figure 2. Mitofilin immunohistochemistry in human cerebellum visualized with mAb MSM02. Mitofilin immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells. Image kindly provided by Nichola Lax and Doug Turnbull, Mitochondrial Research Group, Newcastle University.

WB Images

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Figure 3. Isolated mitochondria from human heart (lane 1), bovine heart (lane 2), rat heart (lane 3), mouse heart (lane 4), and HepG2 (lane 5) detected with (MSM02) anti-mitofilin antibody. Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

Flow Images

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Figure 4. HL-60 cells were stained with 1 µg/mL Mitofilin antibody (MSM02) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.



Downloadable Documents

   Technical Data Sheet

   Western blotting Protocol

   Immunocytochemistry Protocol

   Immunocapture Protocol

   Immunohistochemistry Protocol

   In-Cell ELISA Protocol

   Flow Cytometry Protocol

   MSDS Sodium Azide



Published Studies Using This Product: Keeney et al., 2009. Mitochondrial gene therapy augments mitochondrial physiology in a Parkinson's disease cell model.

Iyer et al., 2009. Recombinant mitochondrial transcription factor A with N-terminal mitochondrial transduction domain increases respiration and mitochondrial gene expression.

Baines et al., 2009. Adenine nucleotide translocase-1 induces cardiomyocyte death through upregulation of the pro-apoptotic protein Bax.

Dave et al., 2008. Assessing mitochondrial outer membrane permeabilization during apoptosis.

Xie et al., 2007. The mitochondrial inner membrane protein mitofilin exists as a complex with SAM50, metaxins 1 and 2, coiled-coil-helix coiled-coil-helix domain-containing protein 3 and 6 and DnaJC11.


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