Mitofilin monoclonal antibody
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Catalog No. MSM02
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$325.00 - 100 µg
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Product Specifications
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Applications:
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Western blotting, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, In-Cell ELISA, Flow Cytometry
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Species Reactivity:
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human
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Host Species:
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mouse
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Isotype:
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IgG1, κ
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Clone ID:
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2E4AD5
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Concentration:
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1.0 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
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Suggested Working Concentration:
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2 µg/mL for Western blotting
1-5 µg/mL for Immunocytochemistry
4 µg/mL for In-Cell ELISA (0.4 µg/well)
1 µg/mL for Flow Cytometry
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Storage Conditions:
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Store at 4°C. Do not freeze.
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Country of Origin:
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USA
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ICC Images
(click to enlarge)
Figure 1. Mitochondrial localization of mitofilin visualized by immunocytochemistry using the anti-mitofilin mAb 2E4AD5 (MSM02). Cultured human fibroblasts were fixed, permeabilized and then labeled with MSM02 followed by a fluorescent goat-anti-mouse IgG.
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IHC Images
(click to enlarge)
Figure 2. Mitofilin immunohistochemistry in human cerebellum visualized with mAb MSM02. Mitofilin immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells. Image kindly provided by Nichola Lax and Doug Turnbull, Mitochondrial Research Group, Newcastle University.
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WB Images
(click to enlarge)
Figure 3. Isolated mitochondria from human heart (lane 1), bovine heart (lane 2), rat heart (lane 3), mouse heart (lane 4), and HepG2 (lane 5) detected with (MSM02) anti-mitofilin antibody. Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
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Flow Images
(click to enlarge)
Figure 4. HL-60 cells were stained with 1 µg/mL Mitofilin antibody (MSM02) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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CART
