Mitochondria Isolation Kit for Cultured Cells



Catalog No. MS852
$185.00
1 Kit without Homogenizer


Catalog No. MS853
$295.00
1 Kit with Homogenizer


Product Description

Contains sufficient reagents for up to 20 benchtop mitochondria isolations from human cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.

Available with a dounce homogenizer as kit MS853.



 
Data
 
Documentation
 
Citations

Product Specifications
 
Kit Components: 50 ml of Reagent A
50 ml of Reagent B
10 ml of Reagent C
2.0 ml Dounce Homogenizer with pestles (included with MS853)
Applications: Mitochondria isolations from cultured cells.
Storage Conditions: Shipped at room temperature.
Upon receipt, store kit at 4°C.
Country of Origin: USA


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Specifications
 
 
Documentation
 
Citations
Figure 1



(click to enlarge)

Figure 1. Isolated mitochondria show enriched signal when compared to the whole cell extract. In lane 1, MRC5 mitochondria isolated with MitoSciences' Benchtop Isolation Kit were loaded at 20 µg. In lanes 2 and 3, post-spin supernatant and whole cell extract were loaded at 20 µg.


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Specifications
 
Data
 
 
Citations

Downloadable Documents


   MS852 Technical Data Sheet

   MS852 Protocol

   MS853 Technical Data Sheet

   MS853 Protocol


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Specifications
 
Data
 
Documentation
 

Published Studies Using This Product: Potula et al., 2010. Methamphetamine Causes Mitrochondrial Oxidative Damage in Human T Lymphocytes Leading to Functional Impairment.

Wang et al., 2009. Localization of age-related macular degeneration-associated ARMS2 in cytosol, not mitochondria.

Oswald et al., 2009. Knockdown of human COX17 affects assembly and supramolecular organization of cytochrome c oxidase.


Have you published studies using this product?
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