Figure 2. An example using MSP31 to quantify PDH levels using various concentration of rat tissue protein extract.

Based on the above standard curve values, 18 µg (control - undiluted), 9 µg (sample A - diluted 1:2) and 1.8 µg (sample B - diluted 1:10) of extract were loaded to each dipstick. Two dipsticks per sample were run (typical intra-assay precision is 90-98%). Using GraphPad software, the signal intensity from the standard curve was interpolated and the quantity of PDH in samples A and B was determined. Based on the above analysis, the unknown samples, A and B, had 48% and 9% of control PDH levels, respectively.

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