Figure 1. The quantity of frataxin captured in each well is proportional to the amount of horseradish peroxidase activity within each well. The quantity is the change in absorbance at 600 nm/minute/amount of sample loaded into the well. Examine the linear rate of increase in absorbance at 600 nm with time. As shown, the rate/slope is calculated between these time points.

For the control or normal sample, the rate versus amount loaded is plotted as a straight line in the linear region of the assay as shown. Compare the rates of the control (normal) sample and with the rate of the null (background) and with your unknowns, experimental or treated samples to get the relative amount of frataxin.

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