Figure 1. An example using MSF31 to quantify frataxin levels from β -lymphocyte cell culture samples derived from control individuals and Friedreich's Ataxia (FA) patients.

Shown is a 1:2 dilution series using a positive control sample. Approximately 6 to 8 dipsticks are suitable for covering the entire working range and the blank for background levels. In this example the dilution series starts with 4 µg of control sample. A one-site hyperbola line was generated for best-fit analysis using GraphPad.

(click to close window)