Figure 2. Immunocytochemistry image of MS716 stained human HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were pretreated with heated antigen retrieval buffer (100 mM Tris, 5% urea, PH 9.5, 95°C, 10 min). The cells were incubated with the antibody (2H6BE7, 5 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in peroxisome.

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