Figure 2. Mitochondrial localization of complex IV visualized by immunocytochemistry using anti-complex IV subunit II mAb 12C4F12 (MS405). Cultured human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with MS405 (5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody (2 µg/ml).

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